Influenza A ns1 Antibody (NS1-23-1): sc-130568

Influenza A ns1 Antibody (NS1-23-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Influenza A ns1 from various Influenza Virus strains including A/Puerto Rico/8/34 (H1N1), A/FPV/Bratislava/79 (H7N7), A/goose/Guangdong/1/96 (H5N1), A/FPV/Rostock/34 (H7N1), A/WSN-HK reassortant (H1N2), A/Victoria/3/75 (H3N2), and A/Thailand/KAN-1/04 (H5N1) by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Influenza A ns1 (NS1-23-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NS1 protein functions as a type I interferon antagonist, proving crucial for viral evasion of host immune response and enhancement of viral replication and pathogenesis. Nuclear localization of NS1 protein enables modulation of cellular RNA transport, splicing, and translation processes. This nuclear positioning allows NS1 protein to interact with host cell factors, influencing antiviral response and supporting efficient viral replication. Understanding NS1 protein′s function and localization remains essential for developing targeted antiviral therapies and advancing knowledge of influenza virus biology.

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Influenza A ns1 Antibody (NS1-23-1) References:

1. Influenza A virus NS1 protein prevents activation of NF-kappaB and induction of alpha/beta interferon.  |  Wang, X., et al. 2000. J Virol. 74: 11566-73. PMID: 11090154
2. Influenza A virus NS2 protein mediates vRNP nuclear export through NES-independent interaction with hCRM1.  |  Neumann, G., et al. 2000. EMBO J. 19: 6751-8. PMID: 11118210
3. Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses bearing the 1918 NS genes.  |  Basler, CF., et al. 2001. Proc Natl Acad Sci U S A. 98: 2746-51. PMID: 11226311
4. Update on influenza and other viral pneumonias.  |  Fleming, DM. and Zambon, M. 2001. Curr Opin Infect Dis. 14: 199-204. PMID: 11979133
5. Interaction of influenza A virus NS1 and cytoskeleton scaffolding protein α-actinin 4.  |  Zeng, Y., et al. 2022. Virus Genes. 58: 15-22. PMID: 34727338
6. How Influenza A Virus NS1 Deals with the Ubiquitin System to Evade Innate Immunity.  |  Lamotte, LA. and Tafforeau, L. 2021. Viruses. 13: PMID: 34835115
7. Influenza A Virus NS1 Protein Structural Flexibility Analysis According to Its Structural Polymorphism Using Computational Approaches.  |  Naceri, S., et al. 2022. Int J Mol Sci. 23: PMID: 35163728
8. Live attenuated influenza A virus vaccines with modified NS1 proteins for veterinary use.  |  Nogales, A., et al. 2022. Front Cell Infect Microbiol. 12: 954811. PMID: 35937688
9. A/(H1N1) pdm09 NS1 promotes viral replication by enhancing autophagy through hijacking the IAV negative regulatory factor LRPPRC.  |  Guo, X., et al. 2023. Autophagy. 19: 1533-1550. PMID: 36300799
10. The circRNA circVAMP3 restricts influenza A virus replication by interfering with NP and NS1 proteins.  |  Min, J., et al. 2023. PLoS Pathog. 19: e1011577. PMID: 37603540
11. Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells.  |  Bougon, J., et al. 2024. J Virol. 98: e0190123. PMID: 38629840
12. The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins.  |  O'Neill, RE., et al. 1998. EMBO J. 17: 288-96. PMID: 9427762

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Influenza A ns1 Antibody (NS1-23-1) has 70 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Influenza A ns1 Antibody (NS1-23-1) Specifications

Alternate Product Name: Influenza A nonstructural (NS) protein 1

Product Clone Name: NS1-23-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ns1

Epitope Species: influenza A virus

Product Reactivity/Detection: ns1

Species Reactivity/Detection: Influenza Virus strains A/Puerto Rico/8/34 (H1N1), A/FPV/Bratislava/79 (H7N7), A/goose/Guangdong/1/96 (H5N1), A/FPV/Rostock/34 (H7N1), A/WSN-HK reassortant (H1N2), A/Victoria/3/75 (H3N2), A/Thailand/KAN-1/04 (H5N1)

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Influenza Virus strains A/Puerto Rico/8/34 (H1N1), A/FPV/Bratislava/79 (H7N7), A/goose/Guangdong/1/96 (H5N1), A/FPV/Rostock/34 (H7N1), A/WSN-HK reassortant (H1N2), A/Victoria/3/75 (H3N2), A/Thailand/KAN-1/04 (H5N1)

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 70 citations in various scientific publications

Available Conjugates/Forms for Influenza A ns1 Antibody (NS1-23-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin