Importin-8 Antibody (C-5): sc-398854
- Importin-8 Antibody (C-5) is a mouse monoclonal IgG1 κ Importin-8 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-59 mapping at the N-terminus of Importin-8 of human origin
- Importin-8 Antibody (C-5) is recommended for detection of Importin-8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Importin-8 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Importin-8 Antibody (C-5) for WB applications. These reagents are now offered in bundles with Importin-8 Antibody (C-5) (see ordering information below).
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Importin-8 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Importin-8 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Importin-8 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Importin-8, also known as IPO8 or RanBP8, is a 1,037 amino acid protein that plays a crucial role in nuclear transport of proteins, particularly those rich in arginine and serine residues. Importin-8 is primarily located in both nucleus and cytoplasm, which enables effective mediation of substrate transport across the nuclear pore complex. As a member of the Importin-β family, Importin-8 acts as a target for Ran, a GTPase, facilitating nuclear protein import. When functioning as an adaptor-like protein, Importin-8 binds to Ran at the nucleoplasmic side of the nuclear pore complex, triggering dissociation of the Importin complex and releasing target substrate into the nucleus. Importin-8 can exist as a heterodimer with karyopherin β1, further underscoring its importance in the intricate process of nuclear transport.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Importin-8 Antibody (C-5) References:
- Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. | Dean, KA., et al. 2001. J Cell Sci. 114: 3479-85. PMID: 11682607
- Compartmentalization directs assembly of the signal recognition particle. | Maity, TS., et al. 2006. Biochemistry. 45: 14955-64. PMID: 17154533
- Validation of endogenous controls for quantitative gene expression analysis: application on brain cortices of human chronic alcoholics. | Johansson, S., et al. 2007. Brain Res. 1132: 20-8. PMID: 17188656
- Preferential utilization of Imp7/8 in nuclear import of Smads. | Yao, X., et al. 2008. J Biol Chem. 283: 22867-74. PMID: 18519565
- Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. | Weinmann, L., et al. 2009. Cell. 136: 496-507. PMID: 19167051
- Importin 8 regulates the transport of mature microRNAs into the cell nucleus. | Wei, Y., et al. 2014. J Biol Chem. 289: 10270-10275. PMID: 24596094
- Importin 8 mediates m7G cap-sensitive nuclear import of the eukaryotic translation initiation factor eIF4E. | Volpon, L., et al. 2016. Proc Natl Acad Sci U S A. 113: 5263-8. PMID: 27114554
- Importin-8 Modulates Division of Apical Progenitors, Dendritogenesis and Tangential Migration During Development of Mouse Cortex. | Nganou, G., et al. 2018. Front Mol Neurosci. 11: 234. PMID: 30042658
- Importin 8 is involved in human periodontitis by the NF-κB pathway. | Du, Y., et al. 2019. Int J Clin Exp Pathol. 12: 711-716. PMID: 31933878
- Transportin: nuclear transport receptor of a novel nuclear protein import pathway. | Nakielny, S., et al. 1996. Exp Cell Res. 229: 261-6. PMID: 8986607
- A novel class of RanGTP binding proteins. | Görlich, D., et al. 1997. J Cell Biol. 138: 65-80. PMID: 9214382
- Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. | Jäkel, S. and Görlich, D. 1998. EMBO J. 17: 4491-502. PMID: 9687515
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Importin-8 Antibody (C-5) | sc-398854 | 200 µg/ml | $316.00 | |||
Importin-8 Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-527825 | 200 µg Ab; 10 µg BP | $354.00 | |||
Importin-8 Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-533198 | 200 µg Ab; 20 µg BP | $354.00 | |||
Importin-8 Antibody (C-5) AC | sc-398854 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Importin-8 Antibody (C-5) HRP | sc-398854 HRP | 200 µg/ml | $316.00 | |||
Importin-8 Antibody (C-5) FITC | sc-398854 FITC | 200 µg/ml | $330.00 | |||
Importin-8 Antibody (C-5) PE | sc-398854 PE | 200 µg/ml | $343.00 | |||
Importin-8 Antibody (C-5) Alexa Fluor® 488 | sc-398854 AF488 | 200 µg/ml | $357.00 | |||
Importin-8 Antibody (C-5) Alexa Fluor® 546 | sc-398854 AF546 | 200 µg/ml | $357.00 | |||
Importin-8 Antibody (C-5) Alexa Fluor® 594 | sc-398854 AF594 | 200 µg/ml | $357.00 | |||
Importin-8 Antibody (C-5) Alexa Fluor® 647 | sc-398854 AF647 | 200 µg/ml | $357.00 | |||
Importin-8 Antibody (C-5) Alexa Fluor® 680 | sc-398854 AF680 | 200 µg/ml | $357.00 | |||
Importin-8 Antibody (C-5) Alexa Fluor® 790 | sc-398854 AF790 | 200 µg/ml | $357.00 |