IMPACT CRISPR/Cas9 KO Plasmid (m): sc-421126
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IMPACT CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IMPACT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IMPACT CRISPR/Cas9 KO Plasmid (m) | sc-421126 | 20 µg | $397.00 |
Overview
IMPACT (inositol monophosphatase domain containing 1) encodes a conserved cytoplasmic protein implicated in translational control and cellular adaptation to nutrient availability. In mammalian cells, IMPACT has been linked to modulation of the integrated stress response, including regulation of the eIF2α pathway and stress-induced translation programs that shape proliferation and survival under metabolic stress. Through these connections, Impact is studied in contexts where proteostasis and amino acid sensing influence neuronal function, development, and stress resilience, providing a molecular entry point for examining how translational checkpoints contribute to disease-relevant phenotypes.
IMPACT CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Impact gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Impact together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Impact open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IMPACT protein expression.
This CRISPR knockout system enables efficient generation of Impact-deficient cell models for investigation of IMPACT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Impact exon(s) critical for IMPACT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Impact genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IMPACT CRISPR/Cas9 KO Plasmid (m) and IMPACT CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Impact locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IMPACT HDR Plasmid (m) and IMPACT HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Impact homology arms to support homology-directed repair at defined Impact target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.