Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IMP-1/IGF2BP1 CRISPR/Cas9 KO Plasmid (m): sc-431212
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IMP-1/IGF2BP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IMP-1/IGF2BP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IMP-1/IGF2BP1 Antibody (D-9): sc-166344
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IMP-1/IGF2BP1 CRISPR/Cas9 KO Plasmid (m) | sc-431212 | 20 µg | $397.00 |
Overview
Igf2bp1 encodes the RNA-binding protein IMP-1/IGF2BP1, a post-transcriptional regulator that recognizes target mRNAs via KH domains and modulates their localization, stability, and translation. In mouse cells, IGF2BP1 helps coordinate programs linked to growth and differentiation by shaping mRNA fate within ribonucleoprotein granules and cytoskeletal transport pathways. Its regulatory network intersects with proliferative and developmental signaling axes and is frequently studied for effects on cell state transitions, migration-associated RNA localization, and translational control. Dysregulated IGF2BP1-associated RNA programs have been implicated in oncogenic phenotypes and altered tissue homeostasis, making it relevant for mechanistic studies of gene expression regulation in disease-relevant models.
IMP-1/IGF2BP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Igf2bp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Igf2bp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Igf2bp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IMP-1/IGF2BP1 protein expression.
This CRISPR knockout system enables efficient generation of Igf2bp1-deficient cell models for investigation of IMP-1/IGF2BP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Igf2bp1 exon(s) critical for IMP-1/IGF2BP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Igf2bp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IMP-1/IGF2BP1 CRISPR/Cas9 KO Plasmid (m) and IMP-1/IGF2BP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Igf2bp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IMP-1/IGF2BP1 HDR Plasmid (m) and IMP-1/IGF2BP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Igf2bp1 homology arms to support homology-directed repair at defined Igf2bp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.