ILT-4 (h): 293T Lysate: sc-115693

ILT-4 (h): 293T Lysate, derived from the HEK 293T cell line, is crucial for the exploration of the Immunoglobulin-like Transcript 4 (ILT-4) in human cellular research, focusing exclusively on its signaling and regulatory functions in immune modulation. The 293T cells, a variant of the original HEK 293, are enhanced with the SV40 large T antigen to facilitate efficient replication and expression of recombinant proteins. This feature is particularly beneficial for studies requiring robust expression of ILT-4, a receptor primarily involved in the regulation of immune responses through its interaction with major histocompatibility complex (MHC) class I molecules. Researchers utilize this lysate to delve into the molecular mechanisms by which ILT-4 modulates signal transduction pathways that influence immune cell activity. Techniques such as co-immunoprecipitation, flow cytometry, and immunofluorescence are employed to study ILT-4's role in inhibiting the activation and maturation of immune cells, providing insights into the non-medical aspects of immune tolerance and surveillance. This research helps to unravel the complex interactions within the immune system, focusing on the basic understanding of cellular communication and regulation.