ILT-4 Antibody (42D1): sc-53594

ILT-4 Antibody (42D1) is a rat monoclonal IgG2a antibody that detects ILT-4 protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). ILT-4 (42D1) antibody is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC). ILT-4, also known as LIR-2, MIR-10, or CD85d antigen, plays a crucial role in immune regulation by competing with CD8A for binding to class I major histocompatibility complex (MHC) antigens, thereby modulating T cell responses. This interaction is vital for maintaining immune homeostasis, as ILT-4 helps prevent excessive activation of T cells, which can lead to autoimmune diseases. ILT-4 is predominantly expressed on monocytes, B cells, dendritic cells, natural killer (NK) cells, and various tissues, including the placenta, lung, and liver. The presence of a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) in ILT-4 allows for initiation of an inhibitory signaling cascade upon ligand binding, further emphasizing ILT-4′s importance in immune regulation. Anti-ILT-4 antibody (42D1) serves as an essential tool for researchers studying immune mechanisms and potential therapeutic targets in immunology.

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ILT-4 Antibody (42D1) References:

1. Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G.  |  Shiroishi, M., et al. 2003. Proc Natl Acad Sci U S A. 100: 8856-61. PMID: 12853576
2. Interleukin 10 regulates cell surface and soluble LIR-2 (CD85d) expression on dendritic cells resulting in T cell hyporesponsiveness in vitro.  |  Beinhauer, BG., et al. 2004. Eur J Immunol. 34: 74-80. PMID: 14971032
3. Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d).  |  Shiroishi, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 16412-7. PMID: 17056715
4. Down-regulation of leucocyte immunoglobulin-like receptor expression in the synovium of rheumatoid arthritis patients after treatment with disease-modifying anti-rheumatic drugs.  |  Huynh, OA., et al. 2007. Rheumatology (Oxford). 46: 742-51. PMID: 17202177
5. Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis.  |  Bergamini, A., et al. 2014. PLoS One. 9: e92018. PMID: 24676037
6. Inhibitory receptor immunoglobulin-like transcript 4 was highly expressed in primary ductal and lobular breast cancer and significantly correlated with IL-10.  |  Liu, J., et al. 2014. Diagn Pathol. 9: 85. PMID: 24762057
7. Co-expression of immunoglobulin-like transcript 4 and angiopoietin-like proteins in human non-small cell lung cancer.  |  Wang, L., et al. 2015. Mol Med Rep. 11: 2789-96. PMID: 25482926
8. First-in-Class Anti-immunoglobulin-like Transcript 4 Myeloid-Specific Antibody MK-4830 Abrogates a PD-1 Resistance Mechanism in Patients with Advanced Solid Tumors.  |  Siu, LL., et al. 2022. Clin Cancer Res. 28: 57-70. PMID: 34598945
9. Tumor-Derived Immunoglobulin-Like Transcript 4 Promotes Postoperative Relapse via Inducing Vasculogenic Mimicry through MAPK/ERK Signaling in Hepatocellular Carcinoma.  |  Li, J., et al. 2024. Am J Pathol. 194: 2398-2411. PMID: 39233275
10. A new human gene complex encoding the killer cell inhibitory receptors and related monocyte/macrophage receptors.  |  Wagtmann, N., et al. 1997. Curr Biol. 7: 615-8. PMID: 9259559
11. A novel immunoglobulin superfamily receptor for cellular and viral MHC class I molecules.  |  Cosman, D., et al. 1997. Immunity. 7: 273-82. PMID: 9285411
12. A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells.  |  Colonna, M., et al. 1997. J Exp Med. 186: 1809-18. PMID: 9382880

The preferred ILT-4/5 antibody is ILT-4/5 Antibody (F-7): sc-390287.