Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ILK Double Nickase Plasmid (h): sc-400864-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ILK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ILK Double Nickase Plasmid (h) and ILK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ILK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ILK Antibody (65.1): sc-20019
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ILK Double Nickase Plasmid (h) | sc-400864-NIC | 20 µg | $410.00 | |||
ILK Double Nickase Plasmid (h2) | sc-400864-NIC-2 | 20 µg | $410.00 |
Integrin-linked kinase (ILK) is a focal adhesion–associated serine/threonine pseudokinase that functions as a scaffold linking β-integrins to the actin cytoskeleton and coordinating mechanotransduction. ILK participates in integrin/FAK signaling and modulates PI3K–AKT, GSK3β/β-catenin, and Rho-family GTPase pathways to regulate cell adhesion, migration, survival, and epithelial–mesenchymal programs. Through its interactions with PINCH and parvin, ILK supports focal adhesion assembly and cytoskeletal remodeling, influencing extracellular matrix sensing and tissue architecture. Dysregulated ILK signaling has been associated with altered invasion, fibrosis-related remodeling, and other pathologies where adhesion-dependent signaling and stress responses are perturbed.
ILK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ILK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ILK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ILK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ILK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.