ILK Antibody (65.1): sc-20019

ILK Antibody (65.1) is a mouse monoclonal IgG2b kappa light chain antibody that detects ILK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ILK Antibody (65.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ILK protein plays a crucial role in cellular signaling and is primarily located at the cell membrane, where ILK interacts with integrins and other signaling molecules. This localization is significant because ILK acts as a critical link between integrins and the actin cytoskeleton, facilitating the transduction of signals that regulate cell adhesion, migration, and survival. By mediating these processes, ILK is involved in various physiological functions, including tissue development and repair, as well as pathological conditions such as cancer progression. The ability of ILK to influence cell behavior underscores ILK′s importance in both normal cellular functions and disease states, making anti-ILK antibody (65.1) an essential tool for researchers studying integrin signaling pathways and their implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ILK Antibody (65.1) References:

1. Integrins: versatility, modulation, and signaling in cell adhesion.  |  Hynes, RO. 1992. Cell. 69: 11-25. PMID: 1555235
2. Role of ERK1/2 and PI3-K in the regulation of CTGF-induced ILK expression in HK-2 cells.  |  Liu, XC., et al. 2007. Clin Chim Acta. 382: 89-94. PMID: 17498677
3. Alphavbeta3 and alphavbeta5 integrins control glioma cell response to ionising radiation through ILK and RhoB.  |  Monferran, S., et al. 2008. Int J Cancer. 123: 357-364. PMID: 18464290
4. Extracellular vesicle-packaged ILK from mesothelial cells promotes fibroblast activation in peritoneal fibrosis.  |  Huang, Q., et al. 2023. J Extracell Vesicles. 12: e12334. PMID: 37357686
5. Endothelial ILK induces cardioprotection by preventing coronary microvascular dysfunction and endothelial-to-mesenchymal transition.  |  Reventun, P., et al. 2023. Basic Res Cardiol. 118: 28. PMID: 37452166
6. An ILK/STAT3 pathway controls glioblastoma stem cell plasticity.  |  Loftus, AEP., et al. 2024. Dev Cell. 59: 3197-3212.e7. PMID: 39326421
7. Integrins and signal transduction pathways: the road taken.  |  Clark, EA. and Brugge, JS. 1995. Science. 268: 233-9. PMID: 7716514
8. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase.  |  Hannigan, GE., et al. 1996. Nature. 379: 91-6. PMID: 8538749
9. Epithelial integrins.  |  Sheppard, D. 1996. Bioessays. 18: 655-60. PMID: 8760339
10. Cooperation between soluble factors and integrin-mediated cell anchorage in the control of cell growth and differentiation.  |  Juliano, R. 1996. Bioessays. 18: 911-7. PMID: 8939069
11. Overexpression of the integrin-linked kinase promotes anchorage-independent cell cycle progression.  |  Radeva, G., et al. 1997. J Biol Chem. 272: 13937-44. PMID: 9153256
12. Integrin-linked protein kinase regulates fibronectin matrix assembly, E-cadherin expression, and tumorigenicity.  |  Wu, C., et al. 1998. J Biol Chem. 273: 528-36. PMID: 9417112

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ILK Antibody (65.1) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-ILK Antibody (65.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ILK Antibody (65.1) Specifications

Alternate Product Name: Integrin linked kinase (ILK)

Product Clone Name: 65.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: ILK -1

Epitope Species: mouse

Product Reactivity/Detection: ILK-1 and ILK-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for ILK Antibody (65.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ILK antibody is ILK Antibody (E-2): sc-137221.