ILBP Antibody (E-9): sc-515609

ILBP Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ILBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ILBP (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ileal lipid binding protein (ILBP) is a member of the fatty acid binding protein (FABP) family, which plays a crucial role in lipid metabolism by binding and transporting long-chain fatty acids. Located in the cytosol of ileocytes, ILBP is essential for the active uptake of bile acids in the ileum, facilitating the transport of dietary lipids from the liver. ILBP′s structure consists of ten antiparallel β strands arranged in two nearly orthogonal β sheets, resembling a β clam shell, and is covered on one side by two short, nearly parallel α helices. This unique configuration allows ILBP to effectively bind fatty acids and bile acids, enhancing affinity for bile acids through a positive-feedback regulation mechanism. The human ILBP gene is located at position 5q35 and is predominantly expressed in the small intestine, underscoring ILBP′s vital role in digestive health and lipid absorption.

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ILBP Antibody (E-9) References:

1. A comparative study of the backbone dynamics of two closely related lipid binding proteins: bovine heart fatty acid binding protein and porcine ileal lipid binding protein.  |  Lücke, C., et al. 1999. Mol Cell Biochem. 192: 109-21. PMID: 10331665
2. Identification of a bile acid-responsive element in the human ileal bile acid-binding protein gene. Involvement of the farnesoid X receptor/9-cis-retinoic acid receptor heterodimer.  |  Grober, J., et al. 1999. J Biol Chem. 274: 29749-54. PMID: 10514450
3. Solution structure of ileal lipid binding protein in complex with glycocholate.  |  Lücke, C., et al. 2000. Eur J Biochem. 267: 2929-38. PMID: 10806391
4. Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure.  |  Kramer, W., et al. 2001. J Biol Chem. 276: 7291-301. PMID: 11069906
5. Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures.  |  Kurz, M., et al. 2003. Proteins. 50: 312-28. PMID: 12486725
6. Human ileal bile acid-binding protein promoter and the effects of CDX2.  |  Barley, NF., et al. 2003. Biochim Biophys Acta. 1630: 138-43. PMID: 14654244
7. Cloning and chromosomal localization of the human ileal lipid-binding protein.  |  Oelkers, P. and Dawson, PA. 1995. Biochim Biophys Acta. 1257: 199-202. PMID: 7619861
8. Flexibility is a likely determinant of binding specificity in the case of ileal lipid binding protein.  |  Lücke, C., et al. 1996. Structure. 4: 785-800. PMID: 8805562
9. Heart-type fatty acid binding protein - involvement in growth inhibition and differentiation.  |  Börchers, T., et al. 1997. Prostaglandins Leukot Essent Fatty Acids. 57: 77-84. PMID: 9250612
10. Intestinal absorption of bile acids: paradoxical behaviour of the 14 kDa ileal lipid-binding protein in differential photoaffinity labelling.  |  Kramer, W., et al. 1998. Biochem J. 333 (Pt 2): 335-41. PMID: 9657973

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ILBP Antibody (E-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ILBP Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ILBP Antibody (E-9) Specifications

Alternate Product Name: ILBP

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-128

Epitope Target: ILBP

Epitope Species: human

Product Reactivity/Detection: ILBP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ILBP Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin