IL-8RA Double Nickase Plasmid (h): sc-401645-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-8RA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-8RA Double Nickase Plasmid (h) and IL-8RA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CXCR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-8RA Antibody (B-1): sc-7303
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-8RA Double Nickase Plasmid (h) | sc-401645-NIC | 20 µg | $410.00 | |||
IL-8RA Double Nickase Plasmid (h2) | sc-401645-NIC-2 | 20 µg | $410.00 |
CXCR1 encodes interleukin-8 receptor alpha (IL-8RA), a seven-transmembrane GPCR that binds ELR+ CXC chemokines such as CXCL8/IL-8 to coordinate neutrophil chemotaxis, adhesion, degranulation, and oxidative burst. Upon ligand engagement, IL-8RA couples to G proteins to activate PLCβ/Ca²⁺ flux, PI3K–AKT, MAPK/ERK, and small GTPase signaling, shaping cytoskeletal remodeling and directed migration. CXCR1 activity integrates with innate immune surveillance and inflammatory cytokine networks, influencing leukocyte trafficking within infected or damaged tissues. Dysregulated CXCR1 signaling has been associated with chronic inflammatory conditions and tumor-associated inflammation through effects on myeloid cell recruitment and inflammatory microenvironments.
IL-8RA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CXCR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CXCR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CXCR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CXCR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.