IL-6 (h2): 293T Lysate: sc-176119
- human IL-6 transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for IL-6 antibodies
- Suitable control antibody: IL-6 Antibody (1): sc-130326 is recommended as control antibody for monitoring of IL-6 expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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IL-6 (h2): 293T Lysate, derived from the HEK 293T cell line, is specifically designed for the study of Interleukin-6 (IL-6) in human cells, focusing on its role within cellular signaling pathways and immune response mechanisms. The 293T cell line, a derivative of the HEK 293 cells, is engineered to contain the SV40 large T antigen, which boosts plasmid replication and protein expression, making it particularly effective for high-yield production of recombinant proteins. This lysate is invaluable for investigating IL-6's involvement in the activation of signal transducer and activator of transcription (STAT) pathways, specifically STAT3. Researchers utilize this lysate to explore how IL-6 influences gene expression related to inflammation and cellular growth, employing techniques such as Western blotting, ELISA, and reporter assays. The research focuses on the non-medical aspects of IL-6 signaling, such as its role in cellular communication and regulation under stress conditions. By analyzing this lysate, scientists gain deeper insights into the fundamental biological processes of cytokine signaling, crucial for understanding the modulation of immune responses and cellular adaptation strategies.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-6 (h2): 293T Lysate | sc-176119 | 100 µg/200 µl | $236.00 |