IL-3/IL-5/GM-CSFRβ Antibody (F-12): sc-393281

IL-3/IL-5/GM-CSFRβ Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects IL-3/IL-5/GM-CSFRβ in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-3/IL-5/GM-CSFRβ (F-12) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IL-3/IL-5/GM-CSF receptors are crucial for hematopoiesis and immune response, mediating cytokine effects on target cells. The common β subunit plays a pivotal role in receptor signaling, being essential for high-affinity binding of unique α subunits to ligands. Mouse β subunits show 91% similarity at amino acid level, yet differ from human β subunit with only 56% homology. This structural divergence may influence receptor functional dynamics and downstream signaling pathways activated upon ligand binding. Mouse and human β subunits, despite lacking intrinsic tyrosine kinase domains, are integral in activating tyrosine phosphorylation-mediated signaling pathways, essential for cellular responses including proliferation and differentiation.

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IL-3/IL-5/GM-CSFRβ Antibody (F-12) References:

1. Opposing effects of sodium salicylate and haematopoietic cytokines IL-3, IL-5 and GM-CSF on mitogen-activated protein kinases and apoptosis of EoL-1 cells.  |  Wong, CK., et al. 1999. Immunol Invest. 28: 365-79. PMID: 10574634
2. Differential regulation of human eosinophil IL-3, IL-5, and GM-CSF receptor alpha-chain expression by cytokines: IL-3, IL-5, and GM-CSF down-regulate IL-5 receptor alpha expression with loss of IL-5 responsiveness, but up-regulate IL-3 receptor alpha expression.  |  Gregory, B., et al. 2003. J Immunol. 170: 5359-66. PMID: 12759409
3. The Shc-binding site of the betac subunit of the GM-CSF/IL-3/IL-5 receptors is a negative regulator of hematopoiesis.  |  Ramshaw, HS., et al. 2007. Blood. 110: 3582-90. PMID: 17638849
4. IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor.  |  Taketazu, F., et al. 1991. J Cell Physiol. 146: 251-7. PMID: 1825663
5. Discovery of IL-5-binding unnatural cyclic peptides from multiple libraries by directed evolution.  |  Fuji, D., et al. 2022. Biochem Biophys Res Commun. 610: 188-195. PMID: 35487180
6. Treatment with interleukin (IL)-5/IL-5 receptor antibodies in patients with severe eosinophilic asthma and COPD.  |  Drick, N., et al. 2022. ERJ Open Res. 8: PMID: 36299360
7. Evidence for a signaling role for the alpha chains of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and IL-5 receptors: divergent signaling pathways between GM-CSF/IL-3 and IL-5.  |  Mire-Sluis, A., et al. 1995. Blood. 86: 2679-88. PMID: 7545466
8. Synergism of IL-3, IL-5, and GM-CSF on eosinophil differentiation and its application for an assay of murine IL-5 as an eosinophil differentiation factor.  |  Takamoto, M. and Sugane, K. 1995. Immunol Lett. 45: 43-6. PMID: 7622186
9. Molecular modeling of the GM-CSF and IL-3 receptor complexes.  |  Lyne, PD., et al. 1995. Protein Sci. 4: 2223-33. PMID: 8535258
10. Similar IL-5, IL-3, and GM-CSF syntheses by eosinophils in the jejunal mucosa of patients with celiac disease and dermatitis herpetiformis.  |  Desreumaux, P., et al. 1998. Clin Immunol Immunopathol. 88: 14-21. PMID: 9683545

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-3/IL-5/GM-CSFRβ Antibody (F-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-3/IL-5/GM-CSFRβ Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-3/IL-5/GM-CSFRβ Antibody (F-12) Specifications

Alternate Product Name: IL-3/IL-5/GM-CSFRβ

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 867-893

Epitope Target: IL-3/IL-5/GM-CSFRβ

Epitope Species: mouse

Product Reactivity/Detection: β chain common to IL-3, IL-5 and GM-CSFR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for IL-3/IL-5/GM-CSFRβ Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IL-3/IL-5/GM-CSFRβ antibody is IL-3/IL-5/GM-CSFRβ Antibody (1C1): sc-21765.