IL-3/IL-5/GM-CSFRβ Antibody (A-3): sc-398246

IL-3/IL-5/GM-CSFRβ Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects IL-3/IL-5/GM-CSFRβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-3/IL-5/GM-CSFRβ Antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The IL-3/IL-5/GM-CSF receptor complex plays a crucial role in hematopoiesis and immune response regulation, as IL-3/IL-5/GM-CSF receptor complex is involved in signaling pathways that promote survival, proliferation, and differentiation of hematopoietic cells. The common β subunit, shared among these receptors, is essential for high-affinity binding of ligands to respective α subunits, facilitating effective signaling. While human and murine β subunits exhibit significant homology, differences in structure and function underscore evolutionary adaptations in receptor signaling mechanisms across species. Understanding interactions and signaling pathways mediated by IL-3/IL-5/GM-CSFRβ complex is vital for developing targeted therapies for various hematological disorders and immune-related diseases.

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IL-3/IL-5/GM-CSFRβ Antibody (A-3) References:

1. Regulation and function of protein kinase B and MAP kinase activation by the IL-5/GM-CSF/IL-3 receptor.  |  Dijkers, PF., et al. 1999. Oncogene. 18: 3334-42. PMID: 10362354
2. Association of RACK1 and PKCbeta with the common beta-chain of the IL-5/IL-3/GM-CSF receptor.  |  Geijsen, N., et al. 1999. Oncogene. 18: 5126-30. PMID: 10490850
3. Structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor common beta-chain bound to an antagonist.  |  Rossjohn, J., et al. 2000. Blood. 95: 2491-8. PMID: 10753826
4. IL-5-Induced JAB-JAK2 interaction.  |  Zahn, S., et al. 2000. Cytokine. 12: 1299-306. PMID: 10975987
5. Critical cytoplasmic domains of the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors for growth signal transduction and tyrosine phosphorylation.  |  Sakamaki, K., et al. 1992. EMBO J. 11: 3541-9. PMID: 1396555
6. Distinguishing between mouse IL-3 and IL-3 receptor-like (IL-5/GM-CSF receptor converter) mRNAs using the polymerase chain reaction method.  |  Fung, MC., et al. 1992. J Immunol Methods. 149: 97-103. PMID: 1583316
7. Differential binding of IL-3 and GM-CSF to human monocytes.  |  Elliott, MJ., et al. 1992. Growth Factors. 6: 15-29. PMID: 1591016
8. Chemoattractant-induced signaling via the Ras-ERK and PI3K-Akt networks, along with leukotriene C4 release, is dependent on the tyrosine kinase Lyn in IL-5- and IL-3-primed human blood eosinophils.  |  Zhu, Y. and Bertics, PJ. 2011. J Immunol. 186: 516-26. PMID: 21106848
9. IL-3 promotes the development of experimental autoimmune encephalitis.  |  Renner, K., et al. 2016. JCI Insight. 1: e87157. PMID: 27734026
10. Targeting IL-5 pathway against airway hyperresponsiveness: A comparison between benralizumab and mepolizumab.  |  Calzetta, L., et al. 2020. Br J Pharmacol. 177: 4750-4765. PMID: 32857420

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-3/IL-5/GM-CSFRβ Antibody (A-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-3/IL-5/GM-CSFRβ Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-3/IL-5/GM-CSFRβ Antibody (A-3) Specifications

Alternate Product Name: IL-3/IL-5/GM-CSFRβ

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-46

Epitope Target: IL-3/IL-5/GM-CSFRβ

Epitope Species: human

Product Reactivity/Detection: 130 kDa β chain common to IL-3R, IL-5R and GM-CSFR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for IL-3/IL-5/GM-CSFRβ Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IL-3/IL-5/GM-CSFRβ antibody is IL-3/IL-5/GM-CSFRβ Antibody (1C1): sc-21765.