IL-2Rβ Double Nickase Plasmid (h): sc-403250-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-2Rβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-2Rβ Double Nickase Plasmid (h) and IL-2Rβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL2RB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-2Rβ Antibody (C-2): sc-166427
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-2Rβ Double Nickase Plasmid (h) | sc-403250-NIC | 20 µg | $410.00 | |||
IL-2Rβ Double Nickase Plasmid (h2) | sc-403250-NIC-2 | 20 µg | $410.00 |
IL2RB encodes the interleukin-2 receptor beta chain (IL-2Rβ; CD122), a shared signaling subunit of the high-affinity IL-2 receptor and the IL-15 receptor complex. Upon cytokine engagement with IL2RA/IL2RG or IL15RA/IL2RG, IL-2Rβ couples to JAK1/JAK3 to activate STAT5 as well as PI3K–AKT and MAPK pathways, shaping transcriptional programs that control lymphocyte survival, proliferation, and differentiation. This signaling axis is central to T cell and NK cell homeostasis, regulatory T cell function, and immune tolerance, making IL2RB a frequent target for studies of immune dysregulation. Altered IL2RB activity or signaling output is relevant to investigations of autoimmunity, immunodeficiency, and inflammatory disease mechanisms in human immune cell models.
IL-2Rβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL2RB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL2RB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL2RB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL2RB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.