IL-21R Double Nickase Plasmid (h): sc-404225-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-21R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-21R Double Nickase Plasmid (h) and IL-21R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL21R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-21R Antibody (H-11): sc-137120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-21R Double Nickase Plasmid (h) | sc-404225-NIC | 20 µg | $410.00 | |||
IL-21R Double Nickase Plasmid (h2) | sc-404225-NIC-2 | 20 µg | $410.00 |
Human IL21R encodes IL-21R, a type I cytokine receptor that pairs with the common γ chain (IL2RG) to mediate IL-21 signaling in lymphoid cells. Ligand engagement activates JAK1/JAK3 and downstream STAT3/STAT1/STAT5 programs, shaping B cell differentiation, germinal center dynamics, antibody class switching, and T cell and NK cell effector functions. IL-21R signaling also interfaces with PI3K–AKT and MAPK pathways to coordinate proliferation, survival, and transcriptional remodeling during immune activation. Dysregulated IL21R activity has been implicated in immune-mediated inflammation, susceptibility to infection, and altered lymphocyte development, making it a relevant node for mechanistic immunology studies.
IL-21R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL21R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL21R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL21R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL21R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.