IL-1Rrp2 CRISPR/Cas9 KO Plasmid (h): sc-406998
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1Rrp2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-1Rrp2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1Rrp2 Antibody (5G5): sc-293377
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1Rrp2 CRISPR/Cas9 KO Plasmid (h) | sc-406998 | 20 µg | $397.00 |
Overview
IL1RL2 encodes IL-1Rrp2, a member of the interleukin-1 receptor family that participates in innate and mucosal immune signaling. As a receptor for IL-36 cytokines, IL-1Rrp2 engages IL-1 receptor accessory protein to drive MyD88-dependent signaling, activating NF-κB and MAPK pathways that regulate inflammatory gene expression and epithelial barrier responses. IL1RL2 is expressed in immune and epithelial compartments and is commonly studied in the context of cytokine-driven inflammation, including skin and airway inflammatory processes. Dysregulated IL-36–IL-1Rrp2 signaling has been associated with inflammatory phenotypes, making this target relevant for mechanistic studies of cytokine networks and tissue inflammation.
IL-1Rrp2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IL1RL2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IL1RL2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IL1RL2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-1Rrp2 protein expression.
This CRISPR knockout system enables efficient generation of IL1RL2-deficient cell models for investigation of IL-1Rrp2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IL1RL2 exon(s) critical for IL-1Rrp2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IL1RL2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-1Rrp2 CRISPR/Cas9 KO Plasmid (h) and IL-1Rrp2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IL1RL2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-1Rrp2 HDR Plasmid (h) and IL-1Rrp2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IL1RL2 homology arms to support homology-directed repair at defined IL1RL2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.