IL-13Rα1 Double Nickase Plasmid (h): sc-405042-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-13Rα1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-13Rα1 Double Nickase Plasmid (h) and IL-13Rα1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL13RA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-13Rα1 Antibody (D-2): sc-398831
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-13Rα1 Double Nickase Plasmid (h) | sc-405042-NIC | 20 µg | $410.00 | |||
IL-13Rα1 Double Nickase Plasmid (h2) | sc-405042-NIC-2 | 20 µg | $410.00 |
IL13RA1 encodes the human interleukin-13 receptor alpha 1 (IL-13Rα1), a type I cytokine receptor subunit that pairs with IL4RA to form a functional receptor for IL-13 and contribute to IL-4 signaling. Ligand engagement activates JAK/STAT pathways, prominently STAT6, driving transcriptional programs involved in epithelial barrier regulation, mucus production, macrophage polarization, and B cell class switching. IL-13Rα1-dependent signaling is central to type 2 inflammation and is frequently studied in airway and skin biology, including models of asthma, allergic inflammation, and atopic dermatitis. Altered IL13RA1 expression or signaling dynamics are also investigated in immune–tumor interactions and fibrotic remodeling contexts where IL-13–STAT6 programs can shape stromal and myeloid phenotypes.
IL-13Rα1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL13RA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL13RA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL13RA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL13RA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.