Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-12B p40 Double Nickase Plasmid (h): sc-401407-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-12B p40 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-12B p40 Double Nickase Plasmid (h) and IL-12B p40 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL12B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-12B p40 Antibody (F-10): sc-365389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-12B p40 Double Nickase Plasmid (h) | sc-401407-NIC | 20 µg | $410.00 | |||
IL-12B p40 Double Nickase Plasmid (h2) | sc-401407-NIC-2 | 20 µg | $410.00 |
IL12B encodes the IL-12B p40 subunit, a shared component of the heterodimeric cytokines IL-12 (p35/p40) and IL-23 (p19/p40) that coordinate innate and adaptive immune responses. Through IL-12 and IL-23 receptor signaling, p40-containing cytokines activate JAK/STAT pathways, promote Th1/Th17 polarization, and support IFN-γ–driven antimicrobial programs and inflammatory gene expression. IL12B expression in antigen-presenting cells is tightly regulated by pattern-recognition receptor inputs, including TLR and NF-κB signaling, linking microbial sensing to cytokine output. Dysregulated IL12B activity has been implicated in chronic inflammatory and autoimmune pathophysiology and in altered host defense, making it a common focus for mechanistic studies in immunology and inflammation models.
IL-12B p40 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL12B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL12B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL12B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL12B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.