IL-12 Antibody (500-M12): sc-65354

IL-12 Antibody (500-M12) is a mouse monoclonal IgG1 antibody that detects interleukin-12 (IL-12) in human samples through applications such as western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-12 is a crucial cytokine produced primarily by macrophages and B-lymphoblastoid cells in response to antigenic stimulation, playing a vital role in the immune response by promoting the differentiation of naive CD4(+) T cells into type 1 helper T cells, which are essential for the production of interferon-γ (IFN-γ). This process is significant as IFN-γ is a key player in enhancing the immune response against intracellular pathogens and tumors. Additionally, IL-12 activates natural killer (NK) cells and stimulates the production of tumor necrosis factor α (TNFα), further amplifying the immune response. The ability of IL-12 to inhibit IL-4 mediated suppression of IFN-γ production underscores its importance in maintaining a balanced immune response. Furthermore, IL-12 exhibits anti-angiogenic properties, as the increased production of IFN-γ leads to the upregulation of inducible protein-10 (IP-10), which can inhibit tumor growth by disrupting the formation of new blood vessels. With its multifaceted roles in immune regulation and potential therapeutic implications, anti-IL-12 antibody (500-M12) is an invaluable tool for researchers studying immune responses and cytokine interactions.

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IL-12 Antibody (500-M12) References:

1. IL10 and IL12B polymorphisms each influence IL-12p70 secretion by dendritic cells in response to LPS.  |  Peng, JC., et al. 2006. Immunol Cell Biol. 84: 227-32. PMID: 16519741
2. Identification of IL18RAP/IL18R1 and IL12B as leprosy risk genes demonstrates shared pathogenesis between inflammation and infectious diseases.  |  Liu, H., et al. 2012. Am J Hum Genet. 91: 935-41. PMID: 23103228
3. IL12B expression is sustained by a heterogenous population of myeloid lineages during tuberculosis.  |  Reeme, AE., et al. 2013. Tuberculosis (Edinb). 93: 343-56. PMID: 23491716
4. Genetic variation at IL12B, IL23R and IL23A is associated with psoriasis severity, psoriatic arthritis and type 2 diabetes mellitus.  |  Eirís, N., et al. 2014. J Dermatol Sci. 75: 167-72. PMID: 24957500
5. LincRNA-Cox2 modulates TNF-α-induced transcription of Il12b gene in intestinal epithelial cells through regulation of Mi-2/NuRD-mediated epigenetic histone modifications.  |  Tong, Q., et al. 2016. FASEB J. 30: 1187-97. PMID: 26578685
6. Relationship between the IL12B (rs3212227) gene polymorphism and susceptibility to multiple autoimmune diseases: A meta-analysis.  |  Li, J., et al. 2016. Mod Rheumatol. 26: 749-56. PMID: 26915668
7. An Infectious Disease-Associated Il12b Polymorphism Regulates IL-12/23 p40 Transcription Involving Poly(ADP-Ribose) Polymerase 1.  |  Zhao, Q., et al. 2017. J Immunol. 198: 2935-2942. PMID: 28219892
8. Polymorphisms of microRNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer.  |  Petkevicius, V., et al. 2017. World J Gastroenterol. 23: 3480-3487. PMID: 28596683
9. Polymeric Nanoparticles that Combine Dexamethasone and Naproxen for the Synergistic Inhibition of Il12b Transcription in Macrophages.  |  Espinosa-Cano, E., et al. 2020. Macromol Biosci. 20: e2000002. PMID: 32421256
10. Low gene expression of TNF, IL17A, IL23A, and IL12B in tumors: A safety surrogate to predict cancer survival associated with biologic therapies.  |  Klebanov, N., et al. 2021. J Am Acad Dermatol. 85: 249-252. PMID: 32822791

The preferred IL-12B p40 antibody is IL-12B p40 Antibody (F-10): sc-365389.