IL-10Rα CRISPR Activation Plasmid (h): sc-402486-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-10Rα CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- IL-10Rα CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by IL-10Rα CRISPR Activation Plasmid (h) and IL-10Rα CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the IL10RA transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-10Rα Antibody (A-3): sc-365374
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-10Rα CRISPR Activation Plasmid (h) | sc-402486-ACT | 20 µg | $397.00 | |||
IL-10Rα CRISPR Activation Plasmid (h2) | sc-402486-ACT-2 | 20 µg | $397.00 |
IL10RA encodes the alpha subunit of the interleukin-10 receptor (IL-10Rα), a high-affinity cytokine-binding component that pairs with IL10RB to form the functional IL-10 receptor complex. Upon IL-10 engagement, the receptor activates JAK1/TYK2 signaling and downstream STAT3-dependent transcriptional programs that constrain inflammatory cytokine production, limit antigen-presenting cell activation, and support tissue-protective immune regulation. IL-10Rα function is therefore central to macrophage and dendritic cell homeostasis, epithelial barrier-associated immune tolerance, and resolution of inflammation. Dysregulation of IL10RA-linked signaling is associated with aberrant immune activation and inflammatory pathology, making it relevant to studies of mucosal immunology, autoimmunity, and host–microbe interactions.
IL-10Rα CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous IL10RA expression without altering the underlying DNA sequence.
IL-10Rα CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the IL10RA locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the IL10RA transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous IL-10Rα expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native IL10RA locus and enabling the study of IL-10Rα-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of IL-10Rα pathway restoration in tumor cells with silenced or reduced IL10RA expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.