IKKγ Antibody (B-3): sc-8032

IKKγ Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect IKKγ, also known as NEMO (NF-κB Essential Modulator), across multiple species including mouse, rat, and human. IKKγ monoclonal antibody (B-3) targets the full-length human IKKγ epitope spanning amino acids 1-419, ensuring comprehensive recognition and reliable performance in various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IKKγ plays a pivotal role in the NF-κB signaling pathway, acting as an essential regulatory subunit of the IKK complex, which is crucial for the phosphorylation and degradation of IκB proteins. This process allows the transcription factor NF-κB to translocate to the nucleus and activate genes involved in immune responses, inflammation, and cell survival, making IKKγ a critical target for studying these fundamental biological mechanisms. The functionality of IKKγ is vital for understanding how cells respond to proinflammatory cytokines and stress signals, highlighting its importance in various physiological and pathological contexts. IKKγ monoclonal antibody (B-3) is available in both non-conjugated and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing researchers with versatile options to suit their specific experimental needs. IKKγ monoclonal antibody (B-3) is recommended for investigating the intricate details of the NF-κB pathway and its implications in diseases such as cancer, autoimmune disorders, and chronic inflammation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IKKγ Antibody (B-3) References:

1. IKK regulation and human genetics.  |  Courtois, G. and Israël, A. 2011. Curr Top Microbiol Immunol. 349: 73-95. PMID: 20845108
2. GSK-3β controls NF-kappaB activity via IKKγ/NEMO.  |  Medunjanin, S., et al. 2016. Sci Rep. 6: 38553. PMID: 27929056
3. IKKγ/NEMO Localization into Multivesicular Bodies.  |  Wackernagel, LM., et al. 2022. Int J Mol Sci. 23: PMID: 35743220
4. Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation.  |  Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
5. NF-kappa B: a lesson in family values.  |  Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
6. CHUK, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain.  |  Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
7. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1.  |  Malinin, NL., et al. 1997. Nature. 385: 540-4. PMID: 9020361
8. Identification and characterization of an IkappaB kinase.  |  Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
9. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB.  |  DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
10. Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor-kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2.  |  Song, HY., et al. 1997. Proc Natl Acad Sci U S A. 94: 9792-6. PMID: 9275204
11. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation.  |  Zandi, E., et al. 1997. Cell. 91: 243-52. PMID: 9346241
12. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation.  |  Yamaoka, S., et al. 1998. Cell. 93: 1231-40. PMID: 9657155

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IKKγ Antibody (B-3) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-IKKγ Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IKKγ Antibody (B-3) Specifications

Alternate Product Name: NEMO

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-419

Epitope Target: IKKγ

Epitope Species: human

Product Reactivity/Detection: IKKγ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for IKKγ Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin