Ihh Double Nickase Plasmid (h): sc-401466-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ihh Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ihh Double Nickase Plasmid (h) and Ihh Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IHH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ihh Antibody (H-12): sc-271101
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ihh Double Nickase Plasmid (h) | sc-401466-NIC | 20 µg | $410.00 | |||
Ihh Double Nickase Plasmid (h2) | sc-401466-NIC-2 | 20 µg | $410.00 |
Indian hedgehog (IHH) encodes Ihh, a secreted morphogen that signals through PTCH1/SMO to activate GLI transcription factors and regulate cell fate decisions during development. Ihh plays central roles in endochondral ossification, chondrocyte proliferation and hypertrophy, and coordination of osteoblast lineage commitment, integrating with pathways that control extracellular matrix production and growth plate organization. In human biology, altered IHH signaling is linked to skeletal patterning defects and dysregulated Hedgehog pathway activity, making it a key node for studying morphogen gradients and tissue homeostasis. As a pathway component with context-dependent outputs, IHH is frequently used to interrogate Hedgehog-driven transcriptional programs and differentiation dynamics.
Ihh Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IHH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IHH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IHH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IHH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.