Ihh Antibody (H-12): sc-271101

Ihh Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ihh protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Ihh antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Indian Hedgehog (Ihh) protein plays a crucial role in embryonic development and tissue patterning, particularly in regulating cell proliferation and differentiation. Ihh is secreted and functions as a signaling molecule that mediates important cell-cell interactions during development. Ihh is part of the hedgehog signaling pathway, which is vital for proper formation of various tissues and organs. Dysregulation of this pathway has been implicated in several developmental disorders and cancers, making Ihh and its interactions essential for understanding these conditions. Ihh undergoes post-translational modifications, including glycosylation, which are important for stability and activity, influencing the ability to interact with target cells and receptors effectively.

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Ihh Antibody (H-12) References:

1. The expressions of the SOX trio, PTHrP (parathyroid hormone-related peptide)/IHH (Indian hedgehog protein) in surgically induced osteoarthritis of the rat.  |  Kim, SY. and Im, GI. 2011. Cell Biol Int. 35: 529-35. PMID: 21073445
2. Knockdown of Indian hedgehog protein induces an inhibition of cell growth and differentiation in osteoblast MC3T3‑E1 cells.  |  Deng, A., et al. 2017. Mol Med Rep. 16: 7987-7992. PMID: 28990069
3. IHH, SHH, and primary cilia mediate epithelial-stromal cross-talk during decidualization in mice.  |  Li, B., et al. 2023. Sci Signal. 16: eadd0645. PMID: 36853961
4. IHH enhancer variant within neighboring NHEJ1 intron causes microphthalmia anophthalmia and coloboma.  |  Wormser, O., et al. 2023. NPJ Genom Med. 8: 22. PMID: 37580330
5. Key Roles of Gli1 and Ihh Signaling in Craniofacial Development.  |  Sun, Q., et al. 2024. Stem Cells Dev. 33: 251-261. PMID: 38623785
6. IHH-GLI-1-HIF-2α signalling influences hypertrophic chondrocytes to exacerbate osteoarthritis progression.  |  Zhang, C., et al. 2024. J Orthop Translat. 49: 207-217. PMID: 39498143
7. Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis.  |  Roelink, H., et al. 1995. Cell. 81: 445-55. PMID: 7736596
8. Long-range sclerotome induction by sonic hedgehog: direct role of the amino-terminal cleavage product and modulation by the cyclic AMP signaling pathway.  |  Fan, CM., et al. 1995. Cell. 81: 457-65. PMID: 7736597
9. Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants.  |  Martí, E., et al. 1995. Nature. 375: 322-5. PMID: 7753196
10. Sonic hedgehog induces the differentiation of ventral forebrain neurons: a common signal for ventral patterning within the neural tube.  |  Ericson, J., et al. 1995. Cell. 81: 747-56. PMID: 7774016
11. Function of protein kinase A in hedgehog signal transduction and Drosophila imaginal disc development.  |  Li, W., et al. 1995. Cell. 80: 553-62. PMID: 7867063
12. Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity.  |  Echelard, Y., et al. 1993. Cell. 75: 1417-30. PMID: 7916661

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ihh Antibody (H-12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Ihh Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ihh Antibody (H-12) Specifications

Alternate Product Name: Indian Hedgehog

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 228-315

Epitope Target: Ihh

Epitope Species: human

Product Reactivity/Detection: Ihh

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Ihh Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin