IGSF4B CRISPR/Cas9 KO Plasmid (h): sc-405765
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGSF4B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IGSF4B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGSF4B CRISPR/Cas9 KO Plasmid (h) | sc-405765 | 20 µg | $397.00 |
Overview
CADM3 (also known as IGSF4B) encodes a cell adhesion molecule of the immunoglobulin superfamily that supports calcium-independent cell–cell contacts and contributes to the organization of membrane microdomains at sites of intercellular junctions. Through adhesion-dependent signaling, CADM3 can influence neurite outgrowth, synaptic connectivity, and epithelial/neuronal tissue architecture, linking it to pathways that govern cell polarity and contact-mediated growth control. Altered expression or regulation of CADM3 has been reported in studies of neurodevelopmental processes and in cancer biology where changes in adhesion programs can impact invasion, differentiation, and metastatic behavior. As a surface-associated adhesion factor, IGSF4B is therefore relevant for investigating how junctional complexes and adhesion signaling reshape cellular communication and tissue organization.
IGSF4B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CADM3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CADM3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CADM3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IGSF4B protein expression.
This CRISPR knockout system enables efficient generation of CADM3-deficient cell models for investigation of IGSF4B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CADM3 exon(s) critical for IGSF4B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CADM3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IGSF4B CRISPR/Cas9 KO Plasmid (h) and IGSF4B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CADM3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IGSF4B HDR Plasmid (h) and IGSF4B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CADM3 homology arms to support homology-directed repair at defined CADM3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.