IgM Fab fragment Antibody (5E673): sc-71330

IgM Fab fragment Antibody (5E673) is a mouse monoclonal IgG2b kappa light chain antibody that detects IgM Fab fragment protein of human origin by enzyme-linked immunosorbent assay (ELISA). Anti-IgM Fab fragment antibody (5E673) is available as a non-conjugated format. Immunoglobulin M (IgM) plays a crucial role in immune response as the first antibody produced by B cells upon encountering an antigen, making IgM essential for early defense against infections. Structurally, IgM exists as a pentamer, consisting of five monomeric units linked together, enhancing IgM′s ability to bind multiple antigens simultaneously. This pentameric form enables a stronger and more effective immune response through cross-linking pathogens and facilitating pathogen clearance. IgM predominantly exists in serum but also functions as a secretory immunoglobulin, providing protection at mucosal surfaces. Monovalent Fab fragments derived from IgM have two antigen-binding sites, enabling versatile applications in research, such as sterically covering immunoglobulin surfaces for double labeling primary antibodies from the same host species or blocking endogenous immunoglobulins in various assays. This functionality enables accurate and reliable results in immunological studies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IgM Fab fragment Antibody (5E673) References:

1. Immunoglobulin heavy chain constant regions regulate immunity and tolerance to idiotypes of antibody variable regions.  |  Reitan, SK. and Hannestad, K. 2002. Proc Natl Acad Sci U S A. 99: 7588-93. PMID: 12032327
2. The primary structure of the Fab fragment of protein KAU, a monoclonal immunoglobulin M cold agglutinin.  |  Leoni, J., et al. 1991. J Biol Chem. 266: 2836-42. PMID: 1993660
3. Monoclonal antibodies specific for murine IgM. II. Activation of B lymphocytes by monoclonal antibodies specific for the four constant domains of IgM.  |  Leptin, M. 1985. Eur J Immunol. 15: 131-7. PMID: 2578964
4. Expression, distribution and specificity of Fc receptors for IgM on murine B cells.  |  Mathur, A., et al. 1988. J Immunol. 141: 1855-62. PMID: 2971716
5. Immunocytochemical Labelling of Haematological Samples Using Monoclonal Antibodies.  |  Erber, WN. 2021. Cells. 11: PMID: 35011689
6. A marmoset T-lymphocyte protein related to defined human serum immunoglobulin and fragments.  |  Marchalonis, JJ. and Wang, AC. 1981. J Immunogenet. 8: 165-75. PMID: 6167643
7. Nucleotide sequence of the constant region of a chicken mu heavy chain immunoglobulin mRNA.  |  Dahan, A., et al. 1983. Nucleic Acids Res. 11: 5381-9. PMID: 6310496
8. Unusual sequences in the murine immunoglobulin mu-delta heavy-chain region.  |  Richards, JE., et al. Nature. 306: 483-7. PMID: 6417547
9. Mapping of heavy chain genes for mouse immunoglobulins M and D.  |  Liu, CP., et al. 1980. Science. 209: 1348-53. PMID: 6774414
10. Determinants of specificity of a baculovirus-expressed antibody Fab fragment that binds selectively to the activated form of integrin alpha IIb beta 3.  |  Abrams, C., et al. 1994. J Biol Chem. 269: 18781-8. PMID: 7518445
11. Direct production of the Fab fragment derived from the sperm immobilizing antibody using polymerase chain reaction and cDNA expression vectors.  |  Sawai, H., et al. 1995. Am J Reprod Immunol. 34: 26-34. PMID: 7576128
12. Modified cytokeratins expressed on the surface of carcinoma cells undergo endocytosis upon binding of human monoclonal antibody and its recombinant Fab fragment.  |  Ditzel, HJ., et al. 1997. Proc Natl Acad Sci U S A. 94: 8110-5. PMID: 9223323

The preferred IgM antibody is IgM Antibody (R1/69): sc-53347.