Anti-IGFBP7 Antibody (H-3) recommended for detection of precursor and mature IGFBP7 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-IGFBP7 Antibody (H-3): sc-365293

Anti-IGFBP7 Antibody (H-3) is rated 4.7 out of 5 by 7.
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Datasheets

    • Anti-IGFBP7 Antibody (H-3) is a mouse monoclonal IgG1 κ IGFBP7 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 181-282 of IGFBP7 of human origin
    • IGFBP7 Antibody (H-3) is recommended for detection of precursor and mature IGFBP7 of human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and bovine
    • Anti-IGFBP7 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IGFBP7 (H-3): sc-365293.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IGFBP7 Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with IGFBP7 Antibody (H-3) (see ordering information below).
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IGFBP7(H-3)抗体可以做COIP吗?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I am using IGFBP7 (H-3): sc-365293 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well in WB experiments WB analysis of IGFBP7 expression in HMEC-1 cell lysate gave specific signal with no background.
Date published: 2021-07-13
Rated 3 out of 5 by from Not very good Tested this antibody in cell lysates and was not able to detect (via western blot) IGFBP7. It works only when i loaded high concentration of proteins
Date published: 2021-01-28
Rated 5 out of 5 by from 抗体很好用! 一抗稀释度适中。在人子宫内膜癌细胞HEC-1-B中检测到的条带特异、清晰,几乎没有背景。销售反馈信息很及时。抗体到货迅速,包装非常仔细。
Date published: 2017-05-05
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including equine, bovine, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from strong signal The strong signal with the Western blot in whole cell lysates of CAKI-1, that can be obtained on baseband. Our test had been successful.
Date published: 2017-01-26
Rated 5 out of 5 by from Produced excellent immunoperoxidase strong Produced excellent immunoperoxidase strong cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Produced positive Western Blot data of IGFBP7 Produced positive Western Blot data of IGFBP7 expression in Caki-1 whole cell lysate. -SCBT QC
Date published: 2013-04-16
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