IGFBP6 CRISPR/Cas9 KO Plasmid (m): sc-421067
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IGFBP6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP6 CRISPR/Cas9 KO Plasmid (m) | sc-421067 | 20 µg | $397.00 |
Overview
Igfbp6 encodes insulin-like growth factor binding protein 6 (IGFBP6), a secreted regulator of insulin-like growth factor signaling with high affinity for IGF-II that modulates ligand bioavailability and receptor activation. By sequestering IGF-II and influencing its distribution in the extracellular space, IGFBP6 can shape PI3K–AKT and MAPK pathway output, thereby impacting proliferation, differentiation, survival, and metabolic adaptation. In mouse tissues, Igfbp6 expression contributes to developmental and tissue homeostasis programs, and altered IGFBP6 activity has been studied in contexts where IGF axis dysregulation is implicated, including cancer biology and cardiometabolic phenotypes. Its extracellular localization also makes it relevant to studies of paracrine communication and microenvironmental control of growth factor signaling.
IGFBP6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Igfbp6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Igfbp6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Igfbp6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IGFBP6 protein expression.
This CRISPR knockout system enables efficient generation of Igfbp6-deficient cell models for investigation of IGFBP6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Igfbp6 exon(s) critical for IGFBP6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Igfbp6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IGFBP6 CRISPR/Cas9 KO Plasmid (m) and IGFBP6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Igfbp6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IGFBP6 HDR Plasmid (m) and IGFBP6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Igfbp6 homology arms to support homology-directed repair at defined Igfbp6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.