IGFBP1 CRISPR Activation Plasmid (h): sc-400915-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- IGFBP1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by IGFBP1 CRISPR Activation Plasmid (h) and IGFBP1 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the IGFBP1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGFBP1 Antibody (H-5): sc-55474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP1 CRISPR Activation Plasmid (h) | sc-400915-ACT | 20 µg | $397.00 | |||
IGFBP1 CRISPR Activation Plasmid (h2) | sc-400915-ACT-2 | 20 µg | $397.00 |
Insulin-like growth factor binding protein 1 (IGFBP1) is a secreted regulator of IGF bioavailability that modulates IGF1/IGF2 signaling by binding ligands and influencing their interaction with IGF receptors. It contributes to endocrine and metabolic homeostasis, integrating cues from nutritional status, insulin signaling, and hepatic transcriptional programs to shape downstream PI3K–AKT and MAPK pathway activity. IGFBP1 also affects cell growth and survival decisions in an IGF-dependent manner and can interact with extracellular matrix components to influence tissue remodeling. Altered IGFBP1 expression has been linked to metabolic dysregulation and is frequently evaluated in studies of liver physiology, inflammation, and cancer-associated signaling contexts.
IGFBP1 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous IGFBP1 expression without altering the underlying DNA sequence.
IGFBP1 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the IGFBP1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the IGFBP1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous IGFBP1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native IGFBP1 locus and enabling the study of IGFBP1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of IGFBP1 pathway restoration in tumor cells with silenced or reduced IGFBP1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.