IGF2BP1/2/3 Antibody (A-2): sc-271785
- IGF2BP1/2/3 Antibody (A-2) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 278-577 mapping at the C-terminus of IGF2BP1 of human origin
- Anti-IGF2BP1/2/3 Antibody (A-2) is recommended for detection of IGF2BP1, IGF2BP2 and IGF2BP3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-IGF2BP1/2/3 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for IGF2BP1/2/3 Antibody (A-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with IGF2BP1/2/3 Antibody (A-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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IGF2BP1/2/3 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects IGF2BP1/2/3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IGF2BP1/2/3 (A-2) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs) play a crucial role in regulating RNA metabolism and synthesis, which is vital for cellular growth and differentiation. IGF2BP1, also known as coding region determinant-binding protein or CRD-BP, is predominantly found in embryonic tissues and is significantly expressed in various cancers, including 81% of colon cancers and 73% of sarcomas. IGF2BP1′s unique structure, which includes a combination of RNA recognition motifs and four heterogeneous nuclear ribonucleoprotein K homology domains, allows binding to specific mRNAs such as c-Myc and IGF-II, thereby influencing their stability and translation. This interaction is particularly important in cancer development, as IGF2BP1 promotes cell proliferation through an IGF-II-dependent mechanism, highlighting potential as a therapeutic target in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IGF2BP1/2/3 Antibody (A-2) References:
- IGF2BP1, IGF2BP2 and IGF2BP3 genotype, haplotype and genetic model studies in metabolic syndrome traits and diabetes. | Rodriguez, S., et al. 2010. Growth Horm IGF Res. 20: 310-8. PMID: 20627640
- IGF2BP1 promotes SRF-dependent transcription in cancer in a m6A- and miRNA-dependent manner. | Müller, S., et al. 2019. Nucleic Acids Res. 47: 375-390. PMID: 30371874
- METTL3 facilitates tumor progression via an m6A-IGF2BP2-dependent mechanism in colorectal carcinoma. | Li, T., et al. 2019. Mol Cancer. 18: 112. PMID: 31230592
- The role of IGF2BP2, an m6A reader gene, in human metabolic diseases and cancers. | Wang, J., et al. 2021. Cancer Cell Int. 21: 99. PMID: 33568150
- Allosteric Regulation of IGF2BP1 as a Novel Strategy for the Activation of Tumor Immune Microenvironment. | Liu, Y., et al. 2022. ACS Cent Sci. 8: 1102-1115. PMID: 36032766
- IGF2BP3 is an essential N6-methyladenosine biotarget for suppressing ferroptosis in lung adenocarcinoma cells. | Xu, X., et al. 2022. Mater Today Bio. 17: 100503. PMID: 36457846
- PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer. | Shi, Y., et al. 2023. Nat Commun. 14: 1932. PMID: 37024475
- m6 A-Dependent Modulation via IGF2BP3/MCM5/Notch Axis Promotes Partial EMT and LUAD Metastasis. | Yang, X., et al. 2023. Adv Sci (Weinh). 10: e2206744. PMID: 37171793
- Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer. | Sun, X., et al. 2023. Clin Transl Med. 13: e1457. PMID: 37877353
- IGF2BP3 promotes mRNA degradation through internal m7G modification. | Liu, C., et al. 2024. Nat Commun. 15: 7421. PMID: 39198433
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGF2BP1/2/3 Antibody (A-2) | sc-271785 | 200 µg/ml | $316.00 | |||
IGF2BP1/2/3 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-551994 | 200 µg Ab; 40 µg BP | $354.00 | |||
IGF2BP1/2/3 Antibody (A-2) AC | sc-271785 AC | 500 µg/ml, 25% agarose | $416.00 | |||
IGF2BP1/2/3 Antibody (A-2) HRP | sc-271785 HRP | 200 µg/ml | $316.00 | |||
IGF2BP1/2/3 Antibody (A-2) FITC | sc-271785 FITC | 200 µg/ml | $330.00 | |||
IGF2BP1/2/3 Antibody (A-2) PE | sc-271785 PE | 200 µg/ml | $343.00 | |||
IGF2BP1/2/3 Antibody (A-2) Alexa Fluor® 488 | sc-271785 AF488 | 200 µg/ml | $357.00 | |||
IGF2BP1/2/3 Antibody (A-2) Alexa Fluor® 546 | sc-271785 AF546 | 200 µg/ml | $357.00 | |||
IGF2BP1/2/3 Antibody (A-2) Alexa Fluor® 594 | sc-271785 AF594 | 200 µg/ml | $357.00 | |||
IGF2BP1/2/3 Antibody (A-2) Alexa Fluor® 647 | sc-271785 AF647 | 200 µg/ml | $357.00 | |||
IGF2BP1/2/3 Antibody (A-2) Alexa Fluor® 680 | sc-271785 AF680 | 200 µg/ml | $357.00 | |||
IGF2BP1/2/3 Antibody (A-2) Alexa Fluor® 790 | sc-271785 AF790 | 200 µg/ml | $357.00 |