Anti-IGF-II Antibody (H-11) recommended for detection of IGF-II of human origin by WB, IP, IF and ELISA

Anti-IGF-II Antibody (H-11): sc-515805

Anti-IGF-II Antibody (H-11) is rated 2.5 out of 5 by 2.
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Datasheets

    • IGF-II Antibody (H-11) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 78-180 of IGF-II of human origin
    • IGF-II Antibody (H-11) is recommended for detection of IGF-II of human origin by WB, IP, IF and ELISA
    • Anti-IGF-II Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IGF-II (H-11): sc-515805.
    • m-IgG1 BP-HRP is the preferred secondary detection reagent for IGF-II Antibody (H-11) for WB applications. This reagent is now offered in a bundle with IGF-II Antibody (H-11) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using IGF-II (H-11): sc-515805?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Works We bought this antibody to test in western blotting, It works, but has a lot of non-specific bands too.
Date published: 2018-12-11
Rated 2 out of 5 by from no signal by IHC-P By IHC-P, there was no signal for trophoblast which have high level of mRNA.
Date published: 2016-12-15
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