IGF-I Antibody (W18) recommended for detection of IGF-I of human origin by WB, IP, IF, IHC(P) and ELISA

IGF-I Antibody (W18): sc-74116

IGF-I Antibody (W18) is rated 4.6 out of 5 by 5.
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Datasheets

    • IGF-I Antibody (W18) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant protein corresponding to amino acids 49-118 of IGF-I of human origin
    • recommended for detection of IGF-I of human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with mouse IGF-I or mouse or human IGF-II
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IGF-I (W18): sc-74116.
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What is the total volume provided of IGF-I (W18): sc-74116 monoclonal antibody?

Asked by: jenni
Each vial contains 100 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and protein stabilizer.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from good result of WB A specific band was detected for IGF-I at about 20kDa (as datasheet indicated),the detect sample is MCF-7cell (loading 60ug),no background.
Date published: 2017-07-02
Rated 5 out of 5 by from western blot we used the IGF-I for western blot ,the result shows strong signal and specific band.I use dilution 1:400.
Date published: 2017-05-13
Rated 5 out of 5 by from Strong stainig Strong stainig of mouse spleen tissue, high expressed,1:500 dilution.
Date published: 2017-05-13
Rated 5 out of 5 by from Specific for IGF-I The W18 clone is highly specific for human IGF-I and will not cross-react with mouse IGF-I, or with IGF-II in human or mouse!
Date published: 2017-01-13
Rated 3 out of 5 by from Robust band detected in WB using human recombinant Robust band detected in WB using human recombinant IGF-I fusion protein. -SCBT QC
Date published: 2015-04-28
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