IgA1 Antibody (H-11) recommended for detection of IgA1 of human origin by WB, IP, IF, IHC(P) and ELISA

IgA1 Antibody (H-11): sc-271913

IgA1 Antibody (H-11) is rated 5.0 out of 5 by 3.
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Datasheets

    • IgA1 Antibody (H-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of IgA1 of human origin
    • IgA1 Antibody (H-11) is recommended for detection of IgA1 of human origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with IgA2
    • IgA1 Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IgA1 (H-11): sc-271913.
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Can IgA1 (H-11): sc-271913 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, IgA1 (H-11): sc-271913 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Endogenous expression in human PBL cells Endogenous expression in human PBL cells, strong signal with some background for WB. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Single strong band for WB in human IgA1 transfected Single strong band for WB in human IgA1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-15
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