IgA₁ Antibody (H-11): sc-271913

IgA1 Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects IgA1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IgA1 monoclonal antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Immunoglobulin A (IgA) plays a crucial role in the mucosal immune system, serving as the first line of defense against pathogens that enter through mucosal surfaces. IgA is primarily produced by B cells located in gut-associated lymphoid tissues, with daily production levels surpassing those of other immunoglobulins. The structure of IgA is particularly important, as IgA typically exists as a dimer, which enhances binding to pathogens and neutralizes them effectively. The dimeric form is stabilized by a joining (J) chain and interacts with the polymeric immunoglobulin receptor (pIgR) for transport across epithelial cells to mucosal surfaces, ensuring strategic positioning where most needed to protect against infections. The heavy chain of IgA is an alpha chain, while the light chains can be either kappa or lambda, contributing to the diversity and specificity of the immune response.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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IgA₁ Antibody (H-11) References:

1. Differentiated B lymphocytes. Potential to express particular antibody variable and constant regions depends on site of lymphoid tissue and antigen load.  |  Gearhart, PJ. and Cebra, JJ. 1979. J Exp Med. 149: 216-27. PMID: 105075
2. The J chain is essential for polymeric Ig receptor-mediated epithelial transport of IgA.  |  Johansen, FE., et al. 2001. J Immunol. 167: 5185-92. PMID: 11673531
3. The carboxyl-terminal domains of IgA and IgM direct isotype-specific polymerization and interaction with the polymeric immunoglobulin receptor.  |  Braathen, R., et al. 2002. J Biol Chem. 277: 42755-62. PMID: 12213814
4. Structural requirements for the interaction of human IgA with the human polymeric Ig receptor.  |  Lewis, MJ., et al. 2005. J Immunol. 175: 6694-701. PMID: 16272325
5. Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells.  |  Mora, JR., et al. 2006. Science. 314: 1157-60. PMID: 17110582
6. [IgA, an essential part of the immune system: selected issues].  |  Czyzewska-Buczyńska, A., et al. 2006. Postepy Hig Med Dosw (Online). 61: 38-47. PMID: 17369772
7. The molecular organization of the protein HC-IgA complex (HC-IgA).  |  Grubb, A., et al. 1986. J Biol Chem. 261: 14313-20. PMID: 2429955
8. Specificity of immunoglobulin heavy chain switch correlates with activity of germline heavy chain genes prior to switching.  |  Stavnezer-Nordgren, J. and Sirlin, S. 1986. EMBO J. 5: 95-102. PMID: 3007121
9. IgA glycosylation and immune complex formation in IgAN.  |  Suzuki, H. and Novak, J. 2021. Semin Immunopathol. 43: 669-678. PMID: 34570260
10. Current understanding of IgA antibodies in the pathogenesis of IgA nephropathy.  |  Nihei, Y., et al. 2023. Front Immunol. 14: 1165394. PMID: 37114051
11. The gut microbiota posttranslationally modifies IgA1 in autoimmune glomerulonephritis.  |  Gleeson, PJ., et al. 2024. Sci Transl Med. 16: eadl6149. PMID: 38536935
12. Human triclonal anti-IgG gammopathy. II. Determination of the antigenic specificity patterns of the IgG, IgA and IgM autoantibodies for the subclasses of IgG.  |  Abraham, GN., et al. 1978. Immunology. 35: 437-45. PMID: 81177

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IgA₁ Antibody (H-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-IgA₁ Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IgA₁ Antibody (H-11) Specifications

Alternate Product Name: IgA1

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: IgA₁

Epitope Species: human

Product Reactivity/Detection: IgA₁

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for IgA₁ Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin