Ig lambda light chain Antibody (mAHuIgL) recommended for detection of Ig λ light chain of human origin by WB, IP and ELISA

Ig λ light chain Antibody (mAHuIgL): sc-73325

Ig λ light chain Antibody (mAHuIgL) is rated 4.0 out of 5 by 3.
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Datasheets

    • Ig λ light chain Antibody (mAHuIgL) is a mouse monoclonal IgG2a (lambda light chain) provided at 100 µg/ml
    • raised against purified Ig λ light chain of human origin
    • recommended for detection of Ig λ light chain of human origin by WB, IP and ELISA
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I would like to use Ig λ light chain mAHuIgL): sc-73325 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: jerojero
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-12
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Rated 4 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Ig light chain protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-18
Rated 4 out of 5 by from Strong band observed by WB using human PBL Strong band observed by WB using human PBL whole cell lysates. -SCBT QC
Date published: 2015-01-17
Rated 4 out of 5 by from Positive detection of human Ig light chain Positive detection of human Ig light chain in NAMALWA cells by WB. -SCBT QC
Date published: 2013-10-19
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