Date published: 2025-10-9

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Ig λ light chain Antibody (A2A-5): sc-69924

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Datasheets
  • Ig λ light chain Antibody (A2A-5) is a mouse monoclonal IgG2a (lambda light chain) provided at 100 µg/ml
  • raised against Ig λ light chain of human origin
  • recommended for detection of Ig λ light chain of human origin by WB, IP and ELISA; non cross-reactive with Ig κ light chain
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ig λ light chain Antibody (A2A-5). This work is in progress.

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    Ig λ light chain Antibody (A2A-5) is a mouse monoclonal IgG1 light chain antibody that detects Ig λ light chain in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Ig λ light chain plays a crucial role in the immune response by forming part of the immunoglobulin structure, which is essential for the recognition and binding of antigens. Ig λ light chain is primarily located in the cytoplasm of B cells, where Ig λ light chain combines with heavy chains to form functional antibodies. Ig λ light chain presence is particularly important because Ig λ light chain contributes to the diversity of the antibody repertoire, allowing the immune system to effectively target a wide range of pathogens. In humans, Ig λ light chain is encoded by genes located on chromosome 22, and Ig λ light chain expression is tightly regulated during B cell development. Ig λ light chain ability to pair with various heavy chains enhances the specificity and affinity of antibodies, making Ig λ light chain vital for effective immune responses. Anti-Ig λ light chain antibody (A2A-5) is an invaluable tool for researchers studying B cell function, antibody production, and the overall dynamics of the immune system.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Ig λ light chain Antibody (A2A-5)

    sc-69924
    100 µg/ml
    $316.00

    What is the recommended starting dilution for Western blot for Ig λ light chain (A2A-5): sc-69924 monoclonal antibody?

    Asked by: AbPolly
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-69924.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-04-05
    • y_2025, m_9, d_25, h_5CST
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    Rated 5 out of 5 by from Positive detection of endogenously expressedPositive detection of endogenously expressed human Ig light chain protein in NAMALWA cells by WB. -SCBT QC
    Date published: 2015-03-14
    Rated 4 out of 5 by from Large band observed by WB corresponding toLarge band observed by WB corresponding to the approximate molecular weight for human Ig light chain using U266 whole cell lysates. -SCBT QC
    Date published: 2015-03-10
    Rated 4 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Ig light chain protein via Western Blot analysis. -SCBT QC
    Date published: 2013-09-12
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    Ig λ light chain Antibody (A2A-5) is rated 4.3 out of 5 by 3.
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