Ig κ chain Antibody (OX20): sc-53080

Ig κ chain Antibody (OX20) is a rat monoclonal IgG2 light chain antibody that detects Ig κ light chains of mouse origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Ig κ chain Antibody (OX20) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. The Ig κ light chain plays a crucial role in immune response, as it pairs with heavy chains to form functional antibodies. This pairing enables proper assembly of immunoglobulins, which are vital for recognizing and neutralizing pathogens. The presence of Ig κ light chain allows for diverse antibody specificities, enabling the immune system to effectively target various antigens. Additionally, the unique Y-shaped configuration formed by two heavy chains and two light chains linked by disulfide bonds is critical for antigen recognition and binding. Understanding Ig κ light chain expression and interactions with heavy chains is fundamental for advancing therapeutic strategies and improving antibody-based applications in research and clinical settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ig κ chain Antibody (OX20) References:

1. Immunoglobulin kappa light chain gene alleles are not associated with primary Sjögren's syndrome.  |  Downie-Doyle, S., et al. 2002. Genes Immun. 3 Suppl 1: S63-5. PMID: 12215905
2. B-cell clonality determination using an immunoglobulin kappa light chain polymerase chain reaction method.  |  Pai, RK., et al. 2005. J Mol Diagn. 7: 300-7. PMID: 15858155
3. Alternative splicing patterns in an aberrantly rearranged immunoglobulin kappa-light-chain gene.  |  Sikder, SK., et al. 1985. Proc Natl Acad Sci U S A. 82: 4045-9. PMID: 2408274
4. Two regulatory elements for immunoglobulin kappa light chain gene expression.  |  Bergman, Y., et al. 1984. Proc Natl Acad Sci U S A. 81: 7041-5. PMID: 6438631
5. Expression of an idiotype (Id-460) during in vivo anti-dinitrophenyl antibody responses. I. Mapping of genes for Id-460 expression to the variable region of immunoglobulin heavy-chain locus and to the variable region of immunoglobulin kappa-light-chain locus.  |  Dzierzak, EA., et al. 1980. J Exp Med. 152: 720-9. PMID: 6774049
6. DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus.  |  Van Ness, BG., et al. 1982. Proc Natl Acad Sci U S A. 79: 262-6. PMID: 6804938
7. Low cytoplasmic mRNA levels of immunoglobulin kappa light chain genes containing nonsense codons correlate with inefficient splicing.  |  Lozano, F., et al. 1994. EMBO J. 13: 4617-22. PMID: 7925302
8. A murine monoclonal multireactive immunoglobulin kappa light chain.  |  Mahana, W., et al. 1994. Scand J Immunol. 39: 107-10. PMID: 8290888
9. Diversity of immunoglobulin kappa light chain gene rearrangements and evidence for somatic mutation in V kappa IV family gene segments in X-linked agammaglobulinemia.  |  Timmers, E., et al. 1993. Eur J Immunol. 23: 619-24. PMID: 8449210
10. Negative regulation of immunoglobulin kappa light-chain gene transcription by a short sequence homologous to the murine B1 repetitive element.  |  Saksela, K. and Baltimore, D. 1993. Mol Cell Biol. 13: 3698-705. PMID: 8497276

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ig κ chain Antibody (OX20) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Ig κ chain Antibody (OX20) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ig κ chain Antibody (OX20) Specifications

Alternate Product Name: Ig κ chain

Product Clone Name: OX20

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₂

Amino Acids:

Epitope Target: IgG₂

Epitope Species: mouse

Product Reactivity/Detection: Ig κ light chains

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG₂ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Ig κ chain Antibody (OX20): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Ig κ chain antibody is Ig κ chain Antibody (L1C1): sc-59265.