Ig J Chain (h): 293T Lysate: sc-115510

Ig J Chain (h): 293T Lysate, derived from the HEK 293T cell line, is instrumental in studying the immunoglobulin J chain in human cells, focusing solely on its scientific applications in immunology research. The HEK 293T cells, a derivative of the original HEK 293 line, are engineered to express the SV40 large T antigen, which enhances plasmid replication and protein production, making them an ideal system for high-efficiency transfection and protein expression studies. This lysate provides a critical resource for investigating the J chain's role in polymeric antibody assembly and secretion, specifically its involvement in linking monomers of IgA and IgM to form dimers and pentamers, respectively. Researchers use this lysate to explore the J chain's influence on the transport and stabilization of these immunoglobulin classes within the secretory pathway of cells, employing techniques such as immunoprecipitation, gel electrophoresis, and Western blotting. By understanding the molecular details of these processes, the lysate aids in deciphering the non-medical aspects of immunoglobulin function, such as their basic structural properties and assembly mechanisms, contributing to fundamental insights into the biophysical and biochemical characteristics of immune molecules.