Ig J chain Antibody (F-12) recommended for detection of Ig J chain of human origin by WB, IP, IF, IHC(P) and ELISA

Ig J chain Antibody (F-12): sc-133177

Ig J chain Antibody (F-12) is rated 5.0 out of 5 by 2.
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Datasheets

    • Ig J chain Antibody (F-12) is a mouse monoclonal IgG2a Ig J chain antibody provided at 200 µg/ml
    • raised against amino acids 23-159 representing full length Ig J chain of human origin
    • Ig J chain Antibody (F-12) is recommended for detection of Ig J chain of human origin by WB, IP, IF, IHC(P) and ELISA
    • Ig J chain Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ig J chain (F-12): sc-133177.
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I am using Ig J chain (F-12): sc-133177 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Nice Western Blot data of Ig J chain expression Nice Western Blot data of Ig J chain expression in non-transfected and human Ig J chain transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-16
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