IFT20 Lentiviral Activation Particles (h): sc-418576-LAC
- Target species: human
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- IFT20 Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- IFT20 Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by IFT20 Lentiviral Activation Plasmid (h) and IFT20 Lentiviral Activation Plasmid (h2) target distinct regulatory regions of the IFT20 promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: IFT20 Antibody (3F3): sc-517184
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFT20 Lentiviral Activation Particles (h) | sc-418576-LAC | 200 µl | $455.00 |
IFT20 (intraflagellar transport 20) is a core component of the intraflagellar transport machinery required for primary cilium assembly and maintenance, supporting cilia-dependent signaling and polarized trafficking. In addition to its ciliary role, IFT20 localizes to the Golgi and participates in vesicle sorting and delivery of membrane proteins to the cilium and immune synapse, linking it to receptor organization and signal transduction. Through these functions, IFT20 influences pathways such as Hedgehog signaling and other cilia-mediated developmental and homeostatic processes. Dysregulated ciliary transport and IFT complex function are broadly associated with ciliopathy-related phenotypes and are also relevant to studies of cell cycle control, differentiation, and tissue morphogenesis.
IFT20 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient IFT20 upregulation across a broader range of human cell types.
IFT20 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the IFT20 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous IFT20 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native IFT20 genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.