IFT20 Antibody (3F3): sc-517184

IFT20 Antibody (3F3) is a mouse monoclonal IgG2a kappa light chain antibody that detects IFT20 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-IFT20 antibody (3F3) is available as the non-conjugated format. IFT20, consisting of 132 amino acids, plays a crucial role in the assembly and maintenance of cilia, which are essential for various cellular processes, including signaling and motility. Located in key cellular structures such as the Golgi apparatus, cilium basal body, and centrosome, IFT20 is involved in the intraflagellar transport (IFT) system, where IFT20 interacts with KIF3B to facilitate the movement of ciliary membrane proteins from the Golgi to the cilium. This transport is vital for proper functioning of cilia, and defects in IFT20 can lead to severe consequences, including misorientation of the mitotic spindle and development of cystic kidney disease, potentially resulting in renal failure. IFT20 is expressed in multiple isoforms and is encoded by a gene located on human chromosome 17, which is significant as chromosome 17 contains over 1,200 genes and represents nearly 2.5% of the human genome.

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IFT20 Antibody (3F3) References:

1. IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia.  |  Baker, SA., et al. 2003. J Biol Chem. 278: 34211-8. PMID: 12821668
2. Cloning and characterization of the human IFT20 gene.  |  Yin, G., et al. 2003. Mol Biol Rep. 30: 255-60. PMID: 14672413
3. Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly.  |  Jurczyk, A., et al. 2004. J Cell Biol. 166: 637-43. PMID: 15337773
4. The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly.  |  Follit, JA., et al. 2006. Mol Biol Cell. 17: 3781-92. PMID: 16775004
5. Intraflagellar transport and functional analysis of genes required for flagellum formation in trypanosomes.  |  Absalon, S., et al. 2008. Mol Biol Cell. 19: 929-44. PMID: 18094047
6. The intraflagellar transport protein IFT57 is required for cilia maintenance and regulates IFT-particle-kinesin-II dissociation in vertebrate photoreceptors.  |  Krock, BL. and Perkins, BD. 2008. J Cell Sci. 121: 1907-15. PMID: 18492793
7. Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease.  |  Jonassen, JA., et al. 2008. J Cell Biol. 183: 377-84. PMID: 18981227

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IFT20 Antibody (3F3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-IFT20 Antibody (3F3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IFT20 Antibody (3F3) Specifications

Alternate Product Name: IFT20

Product Clone Name: 3F3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-148

Epitope Target: IFT20

Epitope Species: human

Product Reactivity/Detection: IFT20

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for IFT20 Antibody (3F3): N/A