Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFRD1 CRISPR/Cas9 KO Plasmid (m): sc-421053
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFRD1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IFRD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IFRD1 Antibody (D-7): sc-515012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFRD1 CRISPR/Cas9 KO Plasmid (m) | sc-421053 | 20 µg | $397.00 |
Overview
Ifrd1 (interferon-related developmental regulator 1; also known as Tis7/PC4) encodes a transcriptional co-regulator that modulates gene expression programs controlling cell differentiation, proliferation, and stress responses. IFRD1 interfaces with chromatin-associated complexes and can influence inflammatory signaling, myogenic and neurodevelopmental pathways, and transcriptional outputs linked to immune cell function. In mouse models, altered Ifrd1 activity has been associated with changes in neutrophil effector responses and tissue repair phenotypes, supporting its relevance in studies of inflammation and degenerative processes. Because IFRD1 impacts context-dependent transcriptional networks, it is frequently investigated in mechanisms of lineage commitment, regeneration, and host-response biology.
IFRD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ifrd1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ifrd1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ifrd1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IFRD1 protein expression.
This CRISPR knockout system enables efficient generation of Ifrd1-deficient cell models for investigation of IFRD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ifrd1 exon(s) critical for IFRD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ifrd1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IFRD1 CRISPR/Cas9 KO Plasmid (m) and IFRD1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ifrd1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IFRD1 HDR Plasmid (m) and IFRD1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ifrd1 homology arms to support homology-directed repair at defined Ifrd1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.