IFN-γ/Interferon gamma Antibody (E-10): sc-373727

IFN-γ Antibody (E-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects interferon gamma (IFN-γ) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IFN-γ (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IFN-γ plays a crucial role in the immune response, acting as an antiviral and antiparasitic agent produced primarily by CD4+/CD8+ lymphocytes and natural killer cells upon activation by antigens, mitogens, or alloantigens. IFN-γ modulates T cell growth and differentiation while inhibiting B cell proliferation, thereby influencing the overall immune response. Various cytokines can induce IFN-γ synthesis, including interleukin-2, fibroblast growth factor, and epidermal growth factor, highlighting IFN-γ′s importance in immune regulation. Structurally, active IFN-γ exists as a homodimer, with each subunit comprising six helices, and this dimeric configuration is stabilized by non-covalent interactions at the helices′ interface. IFN-γ precursor consists of 166 amino acids, which includes a 23 amino acid secretory sequence, and functional diversity is further enhanced by variable glycosylation and the formation of dimers and tetramers under non-denaturing conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IFN-γ/Interferon gamma Antibody (E-10) References:

1. Induction of hair regrowth in the alopecia site of IFN-gamma knockout mice by allografting and IFN-gamma injection into the transplantation site.  |  Hirota, R., et al. 2003. J Interferon Cytokine Res. 23: 433-9. PMID: 13678431
2. Gene expression in IFN-gamma-activated murine macrophages.  |  Pereira, CA., et al. 2004. Braz J Med Biol Res. 37: 1795-809. PMID: 15558186
3. IFN-gamma-dependent regulatory circuits in immune inflammation highlighted in diabetes.  |  Calderon, B., et al. 2008. J Immunol. 181: 6964-74. PMID: 18981116
4. Hydrocortisone inhibits IFN-γ production in equine, ovine, and bovine PBMCs.  |  McCandless, EE., et al. 2013. Vet Immunol Immunopathol. 153: 128-33. PMID: 23351642
5. Lipopolysaccharide induces IFN-γ production in human NK cells.  |  Kanevskiy, LM., et al. 2013. Front Immunol. 4: 11. PMID: 23372571
6. Interaction Between Polymorphisms of IFN-γ and MICA Correlated with Hepatocellular Carcinoma.  |  Li, H., et al. 2016. Med Sci Monit. 22: 549-53. PMID: 26893439
7. Integrated CRISPR-Cas9 System-Mediated Knockout of IFN-γ and IFN-γ Receptor 1 in the Vero Cell Line Promotes Viral Susceptibility.  |  Kim, S., et al. 2022. Int J Mol Sci. 23: PMID: 35897807
8. Transfected Leishmania expressing biologically active IFN-gamma.  |  Tobin, JF., et al. 1993. J Immunol. 150: 5059-69. PMID: 8098724
9. IFN-gamma primes macrophage responses to bacterial DNA.  |  Sweet, MJ., et al. 1998. J Interferon Cytokine Res. 18: 263-71. PMID: 9568729
10. Two families of GTPases dominate the complex cellular response to IFN-gamma.  |  Boehm, U., et al. 1998. J Immunol. 161: 6715-23. PMID: 9862701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IFN-γ/Interferon gamma Antibody (E-10) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-IFN-γ/Interferon gamma Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IFN-γ/Interferon gamma Antibody (E-10) Specifications

Alternate Product Name: IFN-γ

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 25-50

Epitope Target: IFN-γ

Epitope Species: human

Product Reactivity/Detection: precursor and mature IFN-γ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for IFN-γ/Interferon gamma Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IFN-γ/Interferon gamma antibody is IFN-γ/Interferon gamma Antibody (G-23): sc-8423.