IFN-γ/Interferon gamma Antibody (D-3): sc-166800

IFN-γ Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects Interferon (IFN)-γ in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IFN-γ plays a crucial role in the immune response, acting as a potent antiviral and antiparasitic agent produced by activated CD4+/CD8+ lymphocytes and natural killer cells. Anti-IFN-γ antibody (D-3) modulates T cell growth and differentiation while inhibiting B cell proliferation, thereby shaping the adaptive immune response. IFN-γ′s active form is a homodimer, with each subunit comprising six helices, and this dimeric structure is stabilized by non-covalent interactions at the interface of the helices. The precursor of IFN-γ consists of 166 amino acids, which includes a 23 amino acid secretory sequence, and functionality is further enhanced by various post-translational modifications, including glycosylation, which can lead to the formation of multiple isoforms under non-denaturing conditions, such as dimers and tetramers. The versatility of IFN-γ in immune regulation underscores significance in therapeutic applications and research, making IFN-γ (D-3) monoclonal antibody an essential tool for studying immune responses in human samples.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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IFN-γ/Interferon gamma Antibody (D-3) References:

1. Inhibitory effects of IFN-gamma and acyclovir on the glioblastoma cell cycle.  |  Kominsky, SL., et al. 2000. J Interferon Cytokine Res. 20: 463-9. PMID: 10841074
2. The critical role of IFN-gamma in experimental coronavirus retinopathy.  |  Hooks, JJ., et al. 2003. Invest Ophthalmol Vis Sci. 44: 3402-8. PMID: 12882788
3. Elevated plasma levels of interferon (IFN)-gamma, IFN-gamma inducing cytokines, and IFN-gamma inducible CXC chemokines in visceral leishmaniasis.  |  Hailu, A., et al. 2004. Am J Trop Med Hyg. 71: 561-7. PMID: 15569785
4. Post-transcriptional regulation of transferrin receptor mRNA by IFN gamma.  |  Bourgeade, MF., et al. 1992. Nucleic Acids Res. 20: 2997-3003. PMID: 1620595
5. Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis.  |  Quiroga, MF., et al. 2006. J Immunol. 176: 5965-74. PMID: 16670305
6. Immune complexes suppress IFN-gamma signaling by activation of the FcgammaRI pathway.  |  Boekhoudt, GH., et al. 2007. J Leukoc Biol. 81: 1086-92. PMID: 17227821
7. IFN-gamma promoter gene polymorphism in psoriasis vulgaris.  |  Baran, W., et al. 2008. Biomarkers. 13: 52-8. PMID: 17852079
8. IFN-gamma does not mimic the catabolic effects of TNF-alpha.  |  Smith, MA., et al. 2007. Am J Physiol Cell Physiol. 293: C1947-52. PMID: 17928538
9. Tryptophan catabolism restricts IFN-γ-expressing neutrophils and Clostridium difficile immunopathology.  |  El-Zaatari, M., et al. 2014. J Immunol. 193: 807-16. PMID: 24935925
10. Crystallization of the complex of human IFN-gamma and the extracellular domain of the IFN-gamma receptor.  |  Chène, C., et al. 1995. Proteins. 23: 591-4. PMID: 8749856

The preferred IFN-γ/Interferon gamma antibody is IFN-γ/Interferon gamma Antibody (G-23): sc-8423.