IFN-γ/Interferon gamma Antibody (D-3): sc-166800

IFN-γ Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects Interferon (IFN)-γ in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IFN-γ plays a crucial role in the immune response, acting as a potent antiviral and antiparasitic agent produced by activated CD4+/CD8+ lymphocytes and natural killer cells. Anti-IFN-γ antibody (D-3) modulates T cell growth and differentiation while inhibiting B cell proliferation, thereby shaping the adaptive immune response. IFN-γ′s active form is a homodimer, with each subunit comprising six helices, and this dimeric structure is stabilized by non-covalent interactions at the interface of the helices. The precursor of IFN-γ consists of 166 amino acids, which includes a 23 amino acid secretory sequence, and functionality is further enhanced by various post-translational modifications, including glycosylation, which can lead to the formation of multiple isoforms under non-denaturing conditions, such as dimers and tetramers. The versatility of IFN-γ in immune regulation underscores significance in therapeutic applications and research, making IFN-γ (D-3) monoclonal antibody an essential tool for studying immune responses in human samples.

The preferred IFN-γ/Interferon gamma antibody is IFN-γ/Interferon gamma Antibody (G-23): sc-8423.