Anti-IFN-γ/Interferon gamma Antibody (D-3) recommended for detection of precursor and mature IFN-γ of human origin by WB, IP, IF and ELISA

Anti-IFN-γ/Interferon gamma Antibody (D-3): sc-166800

Anti-IFN-γ/Interferon gamma Antibody (D-3) is rated 5.0 out of 5 by 1.
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Datasheets

    • IFN-γ/Interferon gamma Antibody (D-3) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 22-166 of IFN-γ of human origin
    • recommended for detection of precursor and mature IFN-γ of human origin by WB, IP, IF and ELISA
    • See IFN-γ/Interferon gamma (E-10): sc-373727 for IFN-γ/Interferon gamma antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2a BP-HRP is the preferred secondary detection reagent for IFN-γ/Interferon gamma Antibody (D-3) for WB applications. This reagent is now offered in a bundle with IFN-γ/Interferon gamma Antibody (D-3) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-166800: IFN-γ (D-3) monoclonal antibody?

Asked by: Dr Ninau Qelp
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good Western blot data of full Good Western blot data of full-length human recombinant IFN- . -SCBT QC
Date published: 2013-03-24
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