IFN-γ/Interferon gamma Antibody (500-M90): sc-65352
- IFN-γ/Interferon gamma Antibody (500-M90) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against recombinant IFN γ of human origin
- recommended for detection of IFN gamma of human origin by WB, IP and ELISA
- See IFN-γ/Interferon gamma (E-10): sc-373727 for IFN-γ/Interferon gamma antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IFN-γ/Interferon gamma Antibody (500-M90). This work is in progress.
IFN-γ Antibody (500-M90) is a mouse monoclonal IgG1 antibody that detects interferon gamma (IFN-γ) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). IFN-γ plays a crucial role in the immune response, acting as a potent antiviral and antiparasitic agent produced by activated CD4+/CD8+ lymphocytes and natural killer cells. Production of IFN-γ is stimulated by various factors, including interleukin-2, fibroblast growth factor, and epidermal growth factor, which underscores IFN-γ′s importance in modulating T cell growth and differentiation while inhibiting B cell proliferation. IFN-γ exists primarily as a homodimer, with each subunit comprising six helices, and this dimeric structure is essential for biological activity, as IFN-γ is stabilized by non-covalent interactions at the interface of the helices. The precursor of IFN-γ consists of 166 amino acids, including a 23 amino acid secretory sequence, and IFN-γ functionality is further influenced by post-translational modifications such as glycosylation, which can lead to the formation of multiple isoforms under non-denaturing conditions, including dimers and tetramers. The versatility of IFN-γ in immune regulation makes IFN-γ a critical target for research and therapeutic applications, and anti-IFN-γ antibody (500-M90) is an invaluable tool for studying IFN-γ′s role in various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IFN-γ/Interferon gamma Antibody (500-M90) References:
- Activation of protein kinase C delta by IFN-gamma. | Deb, DK., et al. 2003. J Immunol. 171: 267-73. PMID: 12817007
- IL-24 modulates IFN-gamma expression in patients with tuberculosis. | Wu, B., et al. 2008. Immunol Lett. 117: 57-62. PMID: 18199488
- Evaluating pleural ADA, ADA2, IFN-γ and IGRA for diagnosing tuberculous pleurisy. | Keng, LT., et al. 2013. J Infect. 67: 294-302. PMID: 23796864
- An immunofluorescent method for identifying individual IFN-gamma-producing lymphocytes. | Laskay, T., et al. 1986. J Immunol Methods. 95: 1-7. PMID: 2431063
- DCIR maintains bone homeostasis by regulating IFN-γ production in T cells. | Maruhashi, T., et al. 2015. J Immunol. 194: 5681-91. PMID: 25926676
- G2A Signaling Dampens Colitic Inflammation via Production of IFN-γ. | Frasch, SC., et al. 2016. J Immunol. 197: 1425-34. PMID: 27402702
- Development of IFN-γ secretory ELISPOT based assay for screening of ADCC responses. | Shete, A., et al. 2017. J Immunol Methods. 441: 49-55. PMID: 27923642
- Reduced IFN-γ Production in Chronic Brucellosis Patients. | Ghaznavi Rad, E., et al. 2017. Iran J Immunol. 14: 215-222. PMID: 28919584
- Molecular transductional mechanisms by which IFN gamma and other signals regulate macrophage development. | Adams, DO. and Hamilton, TA. 1987. Immunol Rev. 97: 5-27. PMID: 2957307
- The C-terminus of IFN gamma is sufficient for intracellular function. | Szente, BE., et al. 1994. Biochem Biophys Res Commun. 203: 1645-54. PMID: 7945313
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFN-γ/Interferon gamma Antibody (500-M90) | sc-65352 | 100 µg/ml | $322.00 |