IFN- alpha R1 Antibody (MAR1-5A3) recommended for detection of IFN-αR1 of mouse origin by WB, IP, IF, IHC(P) and FCM

IFN-αR1 Antibody (MAR1-5A3): sc-53591

IFN-αR1 Antibody (MAR1-5A3) is rated 4.0 out of 5 by 5.
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Datasheets

    • Anti-IFN-αR1 Antibody (MAR1-5A3) is a mouse monoclonal IgG1 (kappa light chain) IFN-αR1 antibody provided at 200 µg/ml
    • raised against the extracellular domain of IFN-αR1 of mouse origin
    • IFN- alpha R1 Antibody (MAR1-5A3) is recommended for detection of IFN-αR1 of mouse origin by WB, IP, IF, IHC(P) and FCM
    • Anti-IFN- alpha R1 Antibody (MAR1-5A3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for neutralizing, sc-53591 L, 200 µg/0.1 ml
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-αR1 (MAR1-5A3): sc-53591.

Can IFN-αR1 (MAR1-5A3): sc-53591 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IFN-αR1 (MAR1-5A3): sc-53591 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 2 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not show a signal, even at dilution 1:25. Therefore, it does not cross react with human IFN-aR1
Date published: 2018-11-16
Rated 5 out of 5 by from Excellent! This antibody is recommended for detection of IFN-αR1 of mouse origin by WB, IP and FCM.
Date published: 2017-01-26
Rated 5 out of 5 by from IFN-alpha R1,perfect. I useanti- IFN-alpha R1 to study the expression of IFN-alpha R on NK cells,T cells in FCM.The result is perfect.
Date published: 2016-11-20
Rated 4 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for WB on human RD and SK-N-SH cells - SCBT Publication Review
Date published: 2015-02-16
Rated 4 out of 5 by from FACS publishable data with mouse germ cells FACS publishable data with mouse germ cells - SCBT Publication Review
Date published: 2014-01-30
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