IDO (h): 293T Lysate: sc-114136

IDO (h): 293T Lysate, derived from the HEK 293T cell line, is specifically utilized for studying the enzyme Indoleamine 2,3-dioxygenase (IDO) in human cells. This lysate is pivotal for research focused on the enzymatic mechanisms by which IDO modulates tryptophan catabolism, a process crucial for cellular biology research outside of clinical contexts. HEK 293T cells, known for their origin from human embryonic kidney cells and modified to express the SV40 large T antigen, are instrumental in enhancing the replication of plasmids containing the SV40 origin of replication. This feature significantly improves protein yield, making the lysate ideal for experiments requiring high-level expression and subsequent analysis of IDO activity. In research applications, IDO (h): 293T Lysate allows scientists to delve into the biochemical pathways by which IDO influences cellular environment through the kynurenine pathway, assessing its role in cellular stress responses and immune regulation mechanisms. Techniques such as Western blotting, immunofluorescence, and enzyme activity assays are commonly employed to explore IDO's function in modulating these pathways. This research is essential for understanding fundamental biological processes that underpin cellular response mechanisms.