Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IDE Double Nickase Plasmid (h): sc-401900-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IDE Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IDE Double Nickase Plasmid (h) and IDE Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IDE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IDE Antibody (F-9): sc-393887
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IDE Double Nickase Plasmid (h) | sc-401900-NIC | 20 µg | $410.00 | |||
IDE Double Nickase Plasmid (h2) | sc-401900-NIC-2 | 20 µg | $410.00 |
Human IDE (insulin-degrading enzyme) is a zinc-dependent metalloprotease that cleaves insulin and other bioactive peptides, contributing to peptide hormone turnover and proteostasis in the cytosol, endosomes, and peroxisomal compartments. IDE activity influences insulin signaling dynamics and intersects with pathways controlling glucose homeostasis and cellular stress responses. Altered IDE expression or function has been linked to dysregulated insulin clearance and has been studied in the context of metabolic disease biology as well as peptide aggregation processes relevant to neurodegenerative mechanisms. These features make IDE a useful target for investigating how proteolytic regulation shapes endocrine signaling, peptide catabolism, and cellular homeostasis.
IDE Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IDE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IDE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IDE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IDE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.