Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Id4 Double Nickase Plasmid (h): sc-401380-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Id4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Id4 Double Nickase Plasmid (h) and Id4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ID4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Id4 Antibody (B-5): sc-365656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Id4 Double Nickase Plasmid (h) | sc-401380-NIC | 20 µg | $410.00 | |||
Id4 Double Nickase Plasmid (h2) | sc-401380-NIC-2 | 20 µg | $410.00 |
ID4 (inhibitor of DNA binding 4) encodes a helix–loop–helix protein that lacks a DNA-binding domain and modulates transcription by sequestering basic HLH factors, thereby influencing lineage commitment and cellular differentiation programs. In human cells, Id4 is linked to regulation of cell-cycle progression, neurodevelopmental processes, and epithelial differentiation, integrating with broader bHLH-driven transcriptional networks. Altered ID4 expression or epigenetic regulation has been reported across multiple tumor types, where it is associated with changes in proliferation, invasion, and stem-like phenotypes, making it a useful node for mechanistic studies. As a context-dependent regulator, ID4 is frequently examined for its impact on developmental pathways and oncogenic signaling states in cell models.
Id4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ID4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ID4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ID4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ID4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.