Id2 Double Nickase Plasmid (h): sc-400550-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Id2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Id2 Double Nickase Plasmid (h) and Id2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ID2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Id2 Antibody (E-7): sc-398104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Id2 Double Nickase Plasmid (h) | sc-400550-NIC | 20 µg | $410.00 | |||
Id2 Double Nickase Plasmid (h2) | sc-400550-NIC-2 | 20 µg | $410.00 |
Human ID2 (inhibitor of DNA binding 2) encodes a helix–loop–helix protein that lacks a DNA-binding domain and functions as a dominant-negative regulator of E proteins to modulate transcriptional programs controlling proliferation, lineage commitment, and differentiation. Id2 integrates signals from developmental and stress-associated pathways, including TGF-β/BMP and Notch-related networks, influencing cell-cycle control and fate decisions in immune, neural, and epithelial contexts. Dysregulated ID2 expression and activity have been associated with altered differentiation states and aberrant growth phenotypes, making it a useful node for studying mechanisms of cellular plasticity and tumor biology. ID2 is also relevant to research on immune cell development and function, where it helps shape transcriptional circuitry underlying lymphoid and myeloid specification.
Id2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ID2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ID2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ID2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ID2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.