ICAM-1/CD54 Antibody (H-4): sc-390483

ICAM-1 Antibody (H-4) is a mouse monoclonal IgG2a antibody raised against amino acids 396-480 mapping within a C-terminal extracellular domain of human ICAM-1, demonstrating reactivity with mouse, rat, and human species. Anti-ICAM-1 antibody (H-4) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Intercellular adhesion molecule-1 (ICAM-1), also known as CD54, is an integral membrane protein belonging to the immunoglobulin superfamily. Structurally, ICAM-1 consists of five extracellular immunoglobulin-like domains, a single transmembrane domain, and a short cytoplasmic tail. The extracellular domains are crucial for binding to leukocyte integrins such as LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), facilitating cell-cell adhesion during immune responses. The structure of ICAM-1 enables precise interactions necessary for leukocyte adhesion and transmigration across the endothelium during inflammation. Alterations in ICAM-1 structure or expression can impact immune surveillance and are associated with various pathological conditions, including autoimmune diseases and cancer metastasis. Understanding ICAM-1′s structural features is essential for research into its role in immune regulation and potential therapeutic interventions. Anti-ICAM-1 antibody (H-4) provides a valuable tool for studying ICAM-1 expression, localization, and function in physiological and pathological processes.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ICAM-1/CD54 Antibody (H-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-ICAM-1/CD54 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ICAM-1/CD54 Antibody (H-4) Specifications

Alternate Product Name: CD54

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 396-480

Epitope Target: ICAM-1

Epitope Species: human

Product Reactivity/Detection: ICAM-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for ICAM-1/CD54 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ICAM-1/CD54 antibody is ICAM-1/CD54 Antibody (G-5): sc-8439.