Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
I2PP2A Double Nickase Plasmid (h): sc-400881-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- I2PP2A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- I2PP2A Double Nickase Plasmid (h) and I2PP2A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SET. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: I2PP2A Antibody (F-9): sc-133138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
I2PP2A Double Nickase Plasmid (h) | sc-400881-NIC | 20 µg | $410.00 | |||
I2PP2A Double Nickase Plasmid (h2) | sc-400881-NIC-2 | 20 µg | $410.00 |
SET encodes I2PP2A (also known as SET/TAF-Iβ), a multifunctional nuclear phosphoprotein that inhibits protein phosphatase 2A (PP2A) and modulates phosphorylation-dependent signaling. I2PP2A participates in chromatin organization and epigenetic control through interactions with histones and nucleosome assembly factors, influencing transcriptional programs, DNA replication, and DNA damage responses. By constraining PP2A activity, SET impacts MAPK/ERK, AKT, and cell cycle checkpoint pathways, shaping proliferation and stress signaling outputs. Dysregulated SET expression or function has been linked to altered phosphatase balance and chromatin states observed across diverse cancer contexts and other proliferative or genome-instability–associated phenotypes.
I2PP2A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SET locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SET. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SET function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SET-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.